Detail publikace

Electrochemical Study of DNA Damaged by Oxidation Stress

ZÍTKA, O. KŘÍŽKOVÁ, S. SKALIČKOVÁ, S. KOPEL, P. BABULA, P. ADAM, V. KIZEK, R.

Originální název

Electrochemical Study of DNA Damaged by Oxidation Stress

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Many compounds can interact with DNA leading to changes of DNA structure as point mutation and bases excision, which could trigger some metabolic failures, which leads to the changes in DNA structure resulting in cancer. Oxidation of nucleic acid bases belongs to the one of the mostly occurred type of DNA damaging leading to the above mentioned phenomena. The investigation of processes of DNA oxidation damage is topical and electrochemical methods include a versatile and sensitive tool for these purposes. 8-hydroxydeoxyguanosine (8-OHdG) is the most widely accepted marker of DNA damage. Oxidative damage to DNA by free radicals and exposure to ionizing radiation generate several other products within the double helix besides mentioned oxidation products of nucleic acid bases. The basic electrochemical behaviour of nucleic acids bases on various types of carbon electrodes is reviewed. Further, we address our attention on description of oxidation mechanisms and on detection of the most important products of nucleic bases oxidation. The miniaturization of detector coupled with some microfluidic devices is suggested and discussed.

Klíčová slova

Miniaturization; nucleic acid bases; oxidation damage of DNA; voltammetry

Autoři

ZÍTKA, O.; KŘÍŽKOVÁ, S.; SKALIČKOVÁ, S.; KOPEL, P.; BABULA, P.; ADAM, V.; KIZEK, R.

Vydáno

1. 2. 2013

ISSN

1386-2073

Periodikum

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING

Ročník

16

Číslo

2

Stát

Stát Spojené arabské emiráty

Strany od

130

Strany do

141

Strany počet

12

BibTex

@article{BUT143112,
  author="Ondřej {Zítka} and Soňa {Křížková} and Sylvie {Skaličková} and Pavel {Kopel} and Petr {Babula} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Study of DNA Damaged by Oxidation Stress",
  journal="COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING",
  year="2013",
  volume="16",
  number="2",
  pages="130--141",
  issn="1386-2073"
}