Detail publikace

Size-Related Cytotoxicological Aspects of Polyvinylpyrrolidone-Capped Platinum Nanoparticles

BUCHTELOVÁ, H. KYNICKÝ, J. ADAM, P. BRTNICKÝ, M. HEGER, Z. ADAM, V. DOSTÁLOVÁ, S. MICHÁLEK, P. KŘÍŽKOVÁ, S. STRMISKA, V. KOPEL, P. HYNEK, D. RICHTERA, L. RIDOŠKOVÁ, A.

Originální název

Size-Related Cytotoxicological Aspects of Polyvinylpyrrolidone-Capped Platinum Nanoparticles

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

The nanotechnological concept is based on size-dependent properties of particles in the 1-100 nm range. Nevertheless, the connection between their size and effect is still not clear. Thus, we focused on reductive colloidal synthesis, characterization and biological testing of Pt nanoparticles (PtNPs) capped with biocompatible polymer polyvinylpyrrolidone (PVP). Synthesized PtNPs were of 3 different primary sizes (approx. similar to 10; similar to 14 and > 20 nm) and demonstrated exceptional haemocompatibility. In vitro treatment of three different types of malignant cells (prostate - LNCaP, breast - MDA-MB-231 and neuroblastoma - GI-ME-N) revealed that even marginal differences in PtNPs diameter resulted in changes in their cytotoxicity. The highest cytotoxicity was observed using the smallest PtNPs-10, where 24IC(50) was lower (3.1-6.2 mu g/mL) than for cisplatin (8.1-19.8 mu g/mL). In contrast to MDA-MB-231 and LNCaP cells, in - GI-ME-N cells PtNPs caused noticeable changes in their cellular structure without influencing their viability. Post-exposure analyses revealed that PtNPs-29 and PtNPs-40 were capable of forming considerably higher amount of reactive oxygen species with consequent stimulation of expression of metallothionein (MT1/2 and MT3), at both mRNA and protein level. Overall, our pilot study demonstrates that in the nanoscaled world even the smallest differences can have crucial biological effect.

Klíčová slova

Comet assay; Cytotoxicity; Haemocompatibility; Nanomedicine; Reactive oxygen species

Autoři

BUCHTELOVÁ, H.; KYNICKÝ, J.; ADAM, P.; BRTNICKÝ, M.; HEGER, Z.; ADAM, V.; DOSTÁLOVÁ, S.; MICHÁLEK, P.; KŘÍŽKOVÁ, S.; STRMISKA, V.; KOPEL, P.; HYNEK, D.; RICHTERA, L.; RIDOŠKOVÁ, A.

Vydáno

1. 7. 2017

ISSN

0278-6915

Periodikum

FOOD AND CHEMICAL TOXICOLOGY

Ročník

105

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

337

Strany do

346

Strany počet

10

BibTex

@article{BUT138222,
  author="Hana {Michálková} and Simona {Dostálová} and Petr {Michálek} and Soňa {Křížková} and Vladislav {Strmiska} and Pavel {Kopel} and David {Hynek} and Lukáš {Richtera} and Andrea {Ridošková} and Jindřich {Kynický} and Pavlína {Adam} and Martin {Brtnický} and Zbyněk {Heger} and Vojtěch {Adam}",
  title="Size-Related Cytotoxicological Aspects of Polyvinylpyrrolidone-Capped Platinum Nanoparticles",
  journal="FOOD AND CHEMICAL TOXICOLOGY",
  year="2017",
  volume="105",
  number="1",
  pages="337--346",
  doi="10.1016/j.fct.2017.04.043",
  issn="0278-6915"
}