Detail publikace

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Originální název

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Anglický název

Fluorescence method for measuring cell proliferation by image analysis of cell confluency

Jazyk

en

Originální abstrakt

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

Anglický abstrakt

This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol. These parameters are important for quality of migration or perfusion assay experiments in vitro.

BibTex


@inproceedings{BUT137050,
  author="Larisa {Baiazitova} and Josef {Skopalík} and Ondřej {Svoboda} and Jiří {Chmelík} and Ivo {Provazník}",
  title="Fluorescence method for measuring cell proliferation by image analysis of cell confluency",
  annote="This work is based on application image analysis for automatic measuring cell confluency of adherent cells labeled with fluorescent dyes. In this paper an example of segmentation of fibroblasts (3T3 Cells Line) with different levels of confluency is demonstrated. The proposed algorithm can be applied to modified culture chambers and different adherent cell lines. Precise cell enumeration and viability computing can help to optimize coating and culture protocol.  These parameters are important for quality of  migration or perfusion assay experiments in vitro.",
  address="Masarykova Univerzita - MUNI PRESS",
  booktitle="XVII. Workshop of Physical Chemists and Electrochemists",
  chapter="137050",
  edition="1",
  howpublished="online",
  institution="Masarykova Univerzita - MUNI PRESS",
  year="2017",
  month="may",
  pages="47--48",
  publisher="Masarykova Univerzita - MUNI PRESS",
  type="conference paper"
}