Detail publikace

Use of terrestrial gastropods in ecotoxicological research

FOJT, P. ZLÁMALOVÁ GARGOŠOVÁ, H.

Originální název

Use of terrestrial gastropods in ecotoxicological research

Anglický název

Use of terrestrial gastropods in ecotoxicological research

Jazyk

en

Originální abstrakt

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluation of the possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we selected two different life stages of this snail: eggs and juvenile individuals. The assessed substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs and embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the monitored endpoints were: mass and shell diameter. Second part of study - exposition of eggs was performed in small Petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. The exposure of the eggs lasted for 14 days and the percentage of successfully hatched snails was evaluated. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time periods from the start of the test. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of toxicants on the environment.

Anglický abstrakt

The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluation of the possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we selected two different life stages of this snail: eggs and juvenile individuals. The assessed substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs and embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the monitored endpoints were: mass and shell diameter. Second part of study - exposition of eggs was performed in small Petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. The exposure of the eggs lasted for 14 days and the percentage of successfully hatched snails was evaluated. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time periods from the start of the test. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes. The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of toxicants on the environment.

Dokumenty

BibTex


@misc{BUT135702,
  author="Pavel {Fojt} and Helena {Zlámalová Gargošová}",
  title="Use of terrestrial gastropods in ecotoxicological research",
  annote="The main aim of this work is to present the possibility of using a cosmopolitan terrestrial organism and its two different life stages for evaluation of the possible effect of environmental contaminants. The organism used in this study is a pulmonate gastropod Helix aspersa aspersa (syn. Cantareus aspersus or Cornu aspersum), or the brown garden snail in common. For this study, we selected two different life stages of this snail: eggs and juvenile individuals. The assessed substance was cadmium chloride (CdCl2). There were three main parts of the study: standard growth inhibition test with juvenile snails, hatching success test with the eggs and embryotoxicity test. In the first case, the juvenile snails were exposed to different concentrations of cadmium (0 – 500 mg/l) and the toxicant was spiked onto a filter paper on the bottom of the breeding boxes. This test lasted for 28 days and the monitored endpoints were: mass and shell diameter. Second part of study - exposition of eggs was performed in small Petri dishes with a filter paper on the bottom and the concentrations of cadmium were the same as in the first case. The exposure of the eggs lasted for 14 days and the percentage of successfully hatched snails was evaluated. In the last case, we observed the effect of cadmium in a low concentration (6mg/l) on the embryos in different time periods from the start of the test. This test lasted also for 14 days and the endpoints were: size and state of the embryo, morphological and physiological changes.
The results showed significant differences between the sensitivity of eggs and juvenile snails and also negative effects of cadmium chloride on the development of the snail embryos. The present study summarizes a scale of different endpoints, which could help for better understanding the effects of toxicants on the environment.",
  address="Vysoké učení technické v Brně, Fakulta chemická,",
  booktitle="Sborník příspěvků studentská konference chemie je život 2017",
  chapter="135702",
  edition="první",
  howpublished="print",
  institution="Vysoké učení technické v Brně, Fakulta chemická,",
  year="2017",
  month="march",
  pages="152--152",
  publisher="Vysoké učení technické v Brně, Fakulta chemická,",
  type="presentation"
}