Detail publikace

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

MILOSAVLJEVIĆ, V. HADDAD, Y. MERLOS RODRIGO, M. MOULICK, A. POLANSKÁ, H. HYNEK, D. HEGER, Z. KOPEL, P. ADAM, V.

Originální název

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.

Klíčová slova

Zinc-Schiff Base-Novicidin Complex, Prostate Cancer

Autoři

MILOSAVLJEVIĆ, V.; HADDAD, Y.; MERLOS RODRIGO, M.; MOULICK, A.; POLANSKÁ, H.; HYNEK, D.; HEGER, Z.; KOPEL, P.; ADAM, V.

Vydáno

11. 10. 2016

Nakladatel

PLOS

ISSN

1932-6203

Periodikum

PLOS ONE

Ročník

11

Číslo

10

Stát

Spojené státy americké

Strany od

1

Strany do

19

Strany počet

19

URL

http://europepmc.org/articles/PMC5058503?pdf=render

Plný text v Digitální knihovně

http://hdl.handle.net/11012/69382

BibTex

@article{BUT129691,
  author="Vedran {Milosavljević} and Yazan Abdulmajeed Eyadh {Haddad} and Miguel Ángel {Merlos Rodrigo} and Amitava {Moulick} and Hana {Polanská} and David {Hynek} and Zbyněk {Heger} and Pavel {Kopel} and Vojtěch {Adam}",
  title="The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy",
  journal="PLOS ONE",
  year="2016",
  volume="11",
  number="10",
  pages="1--19",
  doi="10.1371/journal.pone.0163983",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5058503?pdf=render"
}