Detail publikace

Palm-Sized Device for Point-of-Care Ebola Detection

NEUŽIL P. , AHRBERG C. D., MANZ A.

Originální název

Palm-Sized Device for Point-of-Care Ebola Detection

Anglický název

Palm-Sized Device for Point-of-Care Ebola Detection

Jazyk

en

Originální abstrakt

We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.

Anglický abstrakt

We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.

Dokumenty

BibTex


@article{BUT128960,
  author="Pavel {Neužil} and Christian D. {Ahrberg} and Andreas {Manz}",
  title="Palm-Sized Device for Point-of-Care Ebola Detection",
  annote="We show the utilization of a recently developed cellphone-sized real-time polymerase chain reaction (PCR) device to detect Ebola virus RNA using single-step reverse transcription PCR (RT-PCR). The device was shown to concurrently perform four PCRs, each with a sample volume of 100 nL: one positive control with both Ebola and GAPDH RNA and one negative control. The last two positions were used to measure the GAPDH and the Ebola content of a sample. A comparison of threshold cycles (CT) from the two samples provided relative quantification. The entire process, which consisted of reverse transcription, PCR amplification, and melting curve analysis (MCA), was conducted in less than 37 min. The next step will be integration with a sample preparation unit to form an integrated sample-to-answer system for point-of-care infectious disease diagnostics.",
  address="American Chemical Society",
  chapter="128960",
  doi="10.1021/acs.analchem.6b00278",
  howpublished="online",
  institution="American Chemical Society",
  number="9",
  volume="88",
  year="2016",
  month="may",
  pages="4803--4807",
  publisher="American Chemical Society",
  type="journal article in Web of Science"
}