Detail publikace

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Originální název

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Anglický název

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Jazyk

en

Originální abstrakt

Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

Anglický abstrakt

Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

BibTex


@article{BUT124267,
  author="Dominika {Kalasová} and Tomáš {Zikmund} and Josef {Jaroš} and Markéta {Tesařová} and Markéta {Kaucká} and Igor {Adameyko} and David {Paloušek} and David {Škaroupka} and Jozef {Kaiser}",
  title="Industrial Tomography System for Answering Biological Issues: Development of the
Mouse Embryo Face.",
  annote="Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens.
Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.",
  address="NDT.net",
  booktitle="6th Conference on Industrial Computed Tomography (ICT) 2016",
  chapter="124267",
  howpublished="online",
  institution="NDT.net",
  number="2016",
  year="2016",
  month="february",
  pages="1--9",
  publisher="NDT.net",
  type="journal article - other"
}