Detail publikace

Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida

HEGER, Z. MICHÁLEK, P. GURÁŇ, R. HAVELKOVÁ, B. KOMÍNKOVÁ, M. CERNEI, N. RICHTERA, L. BEKLOVÁ, M. ADAM, V. KÍZEK, R.

Originální název

Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background The environmental impacts of various substances on all levels of organisms are under investigation. Among these substances, endocrine-disrupting compounds (EDCs) present a threat, although the environmental significance of these compounds remains largely unknown. To shed some light on this field, we assessed the effects of 17 beta-oestradiol on the growth, reproduction and formation of free radicals in Eisenia fetida. Methodology/Principal Findings Although the observed effects on growth and survival were relatively weak, a strong impact on reproduction was observed (50.70% inhibition in 100 mu g/kg of E-2). We further demonstrated that the exposure of the earthworm Eisenia fetida to a contaminant of emerging concern, 17 beta-oestradiol (E2), significantly affected the molecules involved in antioxidant defence. Exposure to E2 results in the production of reactive oxygen species (ROS) and the stimulation of antioxidant systems (metallothionein and reduced oxidized glutathione ratio) but not phytochelatins at both the mRNA and translated protein levels. Matrix-assisted laser desorption/ionization (MALDI)-imaging revealed the subcuticular bioaccumulation of oestradiol- 3,4-quinone, altering the levels of local antioxidants in a time-dependent manner. Conclusions/Significance The present study illustrates that although most invertebrates do not possess oestrogen receptors, these organisms can be affected by oestrogen hormones, likely reflecting free diffusion into the cellular microenvironment with subsequent degradation to molecules that undergo redox cycling, producing ROS, thereby increasing environmental contamination that also perilously affects keystone animals, forming lower trophic levels.

Klíčová slova

DISRUPTING COMPOUNDS; METALLOTHIONEIN; WATER; PHARMACEUTICALS; CONTAMINANTS; EXPRESSION; HORMONES; SOILS; OLIGOCHAETA; POLLUTANTS

Autoři

HEGER, Z.; MICHÁLEK, P.; GURÁŇ, R.; HAVELKOVÁ, B.; KOMÍNKOVÁ, M.; CERNEI, N.; RICHTERA, L.; BEKLOVÁ, M.; ADAM, V.; KÍZEK, R.

Rok RIV

2015

Vydáno

22. 12. 2015

Nakladatel

PLOS

ISSN

1932-6203

Periodikum

PLOS ONE

Ročník

10

Číslo

12

Stát

Spojené státy americké

Strany od

1

Strany do

17

Strany počet

17

URL

http://europepmc.org/articles/PMC4690593?pdf=render

Plný text v Digitální knihovně

http://hdl.handle.net/11012/63710

BibTex

@article{BUT123343,
  author="Zbyněk {Heger} and Petr {Michálek} and Roman {Guráň} and Barbora {Havelková} and Markéta {Komínková} and Natalia Vladimirovna {Cernei} and Lukáš {Richtera} and Miroslava {Beklová} and Vojtěch {Adam} and René {Kizek}",
  title="Exposure to 17 beta-Oestradiol Induces Oxidative Stress in the Non-Oestrogen Receptor Invertebrate Species Eisenia fetida",
  journal="PLOS ONE",
  year="2015",
  volume="10",
  number="12",
  pages="1--17",
  doi="10.1371/journal.pone.0145426",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC4690593?pdf=render"
}