Detail publikace

Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization

MILOSAVLJEVIĆ, V. NGUYEN, H. MICHÁLEK, P. MOULICK, A. KOPEL, P. KIZEK, R. ADAM, V.

Originální název

Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Nanoparticles as a progressively developing branch offer a tool for studying the interaction of carbon quantum dots (CQDs) with DNA. In this study, fluorescent CQDs were synthesized using citric acid covered with polyethylene glycol (PEG) as the source of carbon precursors. Furthermore, interactions between CQDs and DNA (double-stranded DNA and single-stranded DNA) were investigated by spectral methods, gel electrophoresis, and electrochemical analysis. Primarily, the fluorescent behavior of CQDs in the presence of DNA was monitored and major differences in the interaction of CQDs with tested single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) were observed at different amounts of CQDs (μg mL−1: 25, 50, 100, 250, 500). It was found that the interaction of ssDNA with CQDs had no significant influence on the CQDs fluorescence intensity measured at the excitation wavelengths of 280 nm, 350 nm, and 400 nm. However, in the presence of dsDNA, the fluorescence intensity of CQDs was significantly increased. Our results provide basic understanding of the interaction between CQDs and DNA. Such fabricated CQDs–DNA might be of great benefit

Klíčová slova

carbon quantum dots, DNA, spectrophotometry, gel electrophoresis, electrochemical analysis

Autoři

MILOSAVLJEVIĆ, V.; NGUYEN, H.; MICHÁLEK, P.; MOULICK, A.; KOPEL, P.; KIZEK, R.; ADAM, V.

Rok RIV

2015

Vydáno

1. 1. 2015

ISSN

0366-6352

Periodikum

Chemical Papers

Ročník

69

Číslo

1

Stát

Slovenská republika

Strany od

192

Strany do

201

Strany počet

10

BibTex

@article{BUT121158,
  author="Vedran {Milosavljević} and Hoai Viet {Nguyen} and Petr {Michálek} and Amitava {Moulick} and Pavel {Kopel} and René {Kizek} and Vojtěch {Adam}",
  title="Synthesis of carbon quantum dots for DNA labeling and its electrochemical, fluorescent and electrophoretic characterization",
  journal="Chemical Papers",
  year="2015",
  volume="69",
  number="1",
  pages="192--201",
  doi="10.2478/s11696-014-0590-2",
  issn="0366-6352"
}