Detail publikace

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Originální název

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Anglický název

The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart

Jazyk

en

Originální abstrakt

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis.

Anglický abstrakt

Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts. Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis.

BibTex


@inproceedings{BUT118271,
  author="Oto {Janoušek} and Marina {Ronzhina} and Jakub {Hejč} and Veronika {Olejníčková} and Tibor {Stračina} and Kateřina {Fialová} and Marie {Nováková} and Ivo {Provazník} and Jana {Kolářová}",
  title="The Effect of Voltage-Sensitive Dye di-4-ANEPPS on Heart Rate Variability in Langendorff-Perfused Isolated Rabbit Heart",
  annote="Optical mapping of heart electrical activity in Langendorff-perfused isolated hearts is based on voltage-sensitive dyes, of which the most commonly used one is di-4-ANEPPS. Prolongation of action potential duration by di-4-ANEPPS was reported in isolated cells; however, this phenomenon was not observed in the whole heart model. 
In order to search for other adverse phenomena of di-4-ANEPPS use, its effect on heart rate variability (HRV) was investigated during staining and washout periods in five New Zealand White rabbit isolated hearts.
Time domain, frequency domain and non-linear HRV parameters (21 in total) revealed that there are no significant changes between control, staining and washout periods. Voltage-sensitive dye di-4-ANEPPS does not affect HRV when applied into the isolated heart coronary system. It can be concluded that di-4-ANEPPS may be safely used in studies combining optical mapping and HRV analysis. 
",
  booktitle="Computing in Cardiology 2015",
  chapter="118271",
  doi="10.1109/CIC.2015.7411094",
  howpublished="online",
  year="2015",
  month="november",
  pages="1049--1052",
  type="conference paper"
}