Detail publikace

Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

KŘÍŽKOVÁ, S. NGUYEN, H. STANISAVLJEVIC, M. KOPEL, P. VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Originální název

Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

Typ

kapitola v knize

Jazyk

angličtina

Originální abstrakt

The emergence of drug-resistant bacteria and new or changing infectious pathogens is an important public health problem as well as a serious socioeconomic concern. Immunomagnetic separation-based methods create new possibilities for rapidly recognizing many of these pathogens. Nanomaterial-based techniques including fl uorescent labeling by quantum dots as well as immunoextraction by magnetic particles are excellent tools for such purposes. Moreover, the combination with capillary electrophoresis in miniaturized microchip arrangement brings numerous benefi ts such as fast and rapid analysis, low sample consumption, very sensitive electrochemical and fl uorescent detection, portable miniaturized instrumentation, and rapid and inexpensive device fabrication. Here the use of superparamagnetic particle-based fully automated instrumentation to isolate pathogen Staphylococcus aureus and its Zn(II)-containing proteins (Zn-proteins) is reported using a robotic pipetting system speeding up the sample preparation and enabling to analyze 48 real samples within 6 h. Cell lysis and Zn-protein extractions were obtained from a minimum of 100 cells with the suffi cient yield for SDS-PAGE (several tens ng of proteins).

Klíčová slova

Magnetic particles , Immunoseparation , Staphylococcus aureus , Zn-protein

Autoři

KŘÍŽKOVÁ, S.; NGUYEN, H.; STANISAVLJEVIC, M.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

1. 7. 2015

Nakladatel

Springer Science+Business Media New York 2015

ISBN

978-1-4939-2353-3

Kniha

Microchip Capillary Electrophoresis Protocols

Číslo edice

1274

Strany od

67

Strany do

79

Strany počet

13

BibTex

@inbook{BUT115342,
  author="Soňa {Křížková} and Hoai Viet {Nguyen} and Maja {Stanisavljevic} and Pavel {Kopel} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation",
  booktitle="Microchip Capillary Electrophoresis Protocols",
  year="2015",
  publisher="Springer Science+Business Media New York 2015",
  edition="1274",
  pages="67--79",
  doi="10.1007/978-1-4939-2353-3\{_}6,",
  isbn="978-1-4939-2353-3"
}