Detail publikace

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

BALVAN, J. KŘÍŽOVÁ, A. GUMULEC, J. RAUDENSKÁ, M. SLÁDEK, Z. SEDLÁČKOVÁ, M. BABULA, P. SZTALMACHOVÁ, M. KIZEK, R. CHMELÍK, R. MASAŘÍK, M.

Originální název

Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Identification of specific cell death is of a great value for many scientists. Predominant types of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular morphology analysis leads to the misclassification of cell death type due to underestimated oncosis. However, the definition of the oncosis is important because of its potential reversibility. Therefore, FCM analysis of cell death using annexin V/propidium iodide assay was compared with holographic microscopy coupled with fluorescence detection Multimodal holographic microscopy (MHM). The aim was to highlight FCM limitations and to point out MHM advantages. It was shown that the annexin V+/PI phenotype is not specific of early apoptotic cells, as previously believed, and that morphological criteria have to be necessarily combined with annexin V/PI for the cell death type to be ascertained precisely. MHM makes it possible to distinguish oncosis clearly from apoptosis and to stratify the progression of oncosis.

Klíčová slova

Multimodal holographic microscopy; apoptosis; oncosis; necrosis; cell death; plumbagin; flow cytometry; transmission electron microscopy

Autoři

BALVAN, J.; KŘÍŽOVÁ, A.; GUMULEC, J.; RAUDENSKÁ, M.; SLÁDEK, Z.; SEDLÁČKOVÁ, M.; BABULA, P.; SZTALMACHOVÁ, M.; KIZEK, R.; CHMELÍK, R.; MASAŘÍK, M.

Rok RIV

2015

Vydáno

24. 3. 2015

Nakladatel

PLOS

ISSN

1932-6203

Periodikum

PLOS ONE

Ročník

10

Číslo

3

Stát

Spojené státy americké

Strany od

1

Strany do

16

Strany počet

16

URL

https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674

Plný text v Digitální knihovně

http://hdl.handle.net/11012/193259

BibTex

@article{BUT113695,
  author="Jan {Balvan} and Aneta {Křížová} and Jaromír {Gumulec} and Martina {Raudenská} and Zbyšek {Sládek} and Miroslava {Sedláčková} and Petr {Babula} and Markéta {Svobodová} and René {Kizek} and Radim {Chmelík} and Michal {Masařík}",
  title="Multimodal Holographic Microscopy: Distinction between Apoptosis and Oncosis",
  journal="PLOS ONE",
  year="2015",
  volume="10",
  number="3",
  pages="1--16",
  doi="10.1371/journal.pone.0121674",
  issn="1932-6203",
  url="https://journals.plos.org/plosone/article/authors?id=10.1371/journal.pone.0121674"
}