Detail publikace

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

HEGER, Z. CERNEI, N. KŘÍŽKOVÁ, S. MASAŘÍK, M. KOPEL, P. HODEK, P. ZÍTKA, O. ADAM, V. KIZEK, R.

Originální název

Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lex 400 nm). The saturation curve of sarcosine based on FRET efficiency (F604/F510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.

Klíčová slova

CANCER,DIAGNOSTIC MARKERS

Autoři

HEGER, Z.; CERNEI, N.; KŘÍŽKOVÁ, S.; MASAŘÍK, M.; KOPEL, P.; HODEK, P.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

Rok RIV

2015

Vydáno

9. 3. 2015

Nakladatel

Nature Publishing Group

ISSN

2045-2322

Periodikum

Scientific Reports

Ročník

5

Číslo

8868

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

7

Strany počet

7

URL

http://www.nature.com/articles/srep08868

Plný text v Digitální knihovně

http://hdl.handle.net/11012/63720

BibTex

@article{BUT113367,
  author="Zbyněk {Heger} and Natalia Vladimirovna {Cernei} and Soňa {Křížková} and Michal {Masařík} and Pavel {Kopel} and Petr {Hodek} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection",
  journal="Scientific Reports",
  year="2015",
  volume="5",
  number="8868",
  pages="1--7",
  doi="10.1038/srep08868",
  issn="2045-2322",
  url="http://www.nature.com/articles/srep08868"
}