Detail publikace

Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene

NGUYEN, H. TMEJOVÁ, K. KREJČOVÁ, L. HYNEK, D. KOPEL, P. KYNICKÝ, J. ADAM, V. KIZEK, R.

Originální název

Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

This paper comes with the application of the Brdicka method for PNA testing and characterization. The classical approach based on the catalytic electroreduction of protons was used. In this study electrochemical determination of PNA in Brdicka solution was investigated by two techniques of differential pulse voltammetry (DPV) and DPV coupled with adsorptive stripping technique. The instrumentation of experiment was improved by manufacturing new holder for cooling electrochemical cell necessary for measurements in temperature sensitive electrolyte. This holder was made using 3D printing system from polylactide (PLA), biodegradable thermoplastic aliphatic polyester. AdT DPV produced four typical peaks (Co1 peak at potential -1.00 + - 0.05 V, RS2CO at potential -1.25 + - 0.05 V, Cat1 at potential -1.35 + - 0.05 V and Cat2 at potential -1.55 + - 0.05 V). Cat2 peak height was strongly depended on accumulation time, temperature of electrolyte, and concentration of PNA

Klíčová slova

PNA; Brdicka Electrolyte; 3D printing; Voltammetry; Adsorptive Transfer Technique

Autoři

NGUYEN, H.; TMEJOVÁ, K.; KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; KYNICKÝ, J.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 7. 2014

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

9

Číslo

7

Stát

Srbská republika

Strany od

3364

Strany do

3373

Strany počet

10

BibTex

@article{BUT109362,
  author="Hoai Viet {Nguyen} and Kateřina {Tmejová} and Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Jindřich {Kynický} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Characterization of PNA Oligonucleotide of Neuraminidase Gene",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3364--3373",
  issn="1452-3981"
}