Detail publikace

Electrochemical fabrication of optical biosensor for DNA detection

Originální název

Electrochemical fabrication of optical biosensor for DNA detection

Anglický název

Electrochemical fabrication of optical biosensor for DNA detection

Jazyk

en

Originální abstrakt

The work is focused on quantum dots (QDs) array fabrication from non-toxic titanium dioxide using template based non-lithographic method, their luminescence properties study and finally their usage for DNA optical detection in situ. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via avidin-biotin molecules and further with single stranded DNA (ssDNA) or oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.

Anglický abstrakt

The work is focused on quantum dots (QDs) array fabrication from non-toxic titanium dioxide using template based non-lithographic method, their luminescence properties study and finally their usage for DNA optical detection in situ. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via avidin-biotin molecules and further with single stranded DNA (ssDNA) or oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.

Dokumenty

BibTex


@inproceedings{BUT93844,
  author="Jiřina {Valkovičová} and Radim {Hrdý} and Jana {Drbohlavová} and Jaromír {Hubálek}",
  title="Electrochemical fabrication of optical biosensor for DNA detection",
  annote="The work is focused on quantum dots (QDs) array fabrication from non-toxic titanium dioxide using template based non-lithographic method, their luminescence properties study and finally their usage for DNA optical detection in situ. The paper describes TiO2 QDs surface modification with gold using suitable template technique and subsequent functionalization of gold modified QDs surface via avidin-biotin molecules and further with single stranded DNA (ssDNA) or oligonucleotides. This functinalization step provides a suitable base for subsequent hybridization reaction with detected nucleic acid. The system of biosensor (TiO2 QDs modified with gold and biomolecules) is designed for rapid optical diagnosis of viral diseases.",
  address="Mendelova univerzita v Brně",
  booktitle="XII. Pracovní setkání fyzikálních chemiků a elektrochemiků",
  chapter="93844",
  howpublished="print",
  institution="Mendelova univerzita v Brně",
  year="2012",
  month="may",
  pages="314--316",
  publisher="Mendelova univerzita v Brně",
  type="conference paper"
}