Detail publikace

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

Originální název

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

Anglický název

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

Jazyk

en

Originální abstrakt

Viral infections pose a threat for man kind. Diagnostic systems focus on the direct cultural proof of identity. Almost all systems use polymerase chain reaction. Real-time polymerase chain reaction is based on the polymerase chain reaction and routinely used to amplify and simultaneously quantify a targeted DNA molecule. Fluorescent dyes intercalating with double-stranded DNA and modified DNA oligonucleotide probes fluorescing when hybridized with a complementary DNA are used for real time quantification of the amplicons. The process of DNA quantification has demands on robust instrument and on high cost chemicals. Electrochemical detection is low cost and sensitive alternative method to detect nucleic acids. The main aim of this work is to propose novel electrochemical detector for monitoring PCR.

Anglický abstrakt

Viral infections pose a threat for man kind. Diagnostic systems focus on the direct cultural proof of identity. Almost all systems use polymerase chain reaction. Real-time polymerase chain reaction is based on the polymerase chain reaction and routinely used to amplify and simultaneously quantify a targeted DNA molecule. Fluorescent dyes intercalating with double-stranded DNA and modified DNA oligonucleotide probes fluorescing when hybridized with a complementary DNA are used for real time quantification of the amplicons. The process of DNA quantification has demands on robust instrument and on high cost chemicals. Electrochemical detection is low cost and sensitive alternative method to detect nucleic acids. The main aim of this work is to propose novel electrochemical detector for monitoring PCR.

BibTex


@article{BUT93578,
  author="Vojtěch {Adam} and Dalibor {Húska} and Soňa {Křížková} and Jaromír {Hubálek} and René {Kizek}",
  title="Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses",
  annote="Viral infections pose a threat for man kind. Diagnostic systems focus on the direct cultural proof of identity. Almost all systems use polymerase chain reaction. Real-time polymerase chain reaction is based on the polymerase chain reaction and routinely used to amplify and simultaneously quantify a targeted DNA molecule. Fluorescent dyes intercalating with double-stranded DNA and modified DNA oligonucleotide probes fluorescing when hybridized with a complementary DNA are used for real time quantification of the amplicons. The process of DNA quantification has demands on robust instrument and on high cost chemicals. Electrochemical detection is low cost and sensitive alternative method to detect nucleic acids. The main aim of this work is to propose novel electrochemical detector for monitoring PCR.",
  address="FEBS JOURNAL",
  chapter="93578",
  institution="FEBS JOURNAL",
  number="Suppl. 1",
  volume="276",
  year="2009",
  month="july",
  pages="95--96",
  publisher="FEBS JOURNAL",
  type="journal article in Web of Science"
}