Detail publikace

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

ADAM,V. HÚSKA,D. KŘÍŽKOVÁ,S.HUBÁLEK,J.KIZEK,R.

Originální název

Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Viral infections pose a threat for man kind. Diagnostic systems focus on the direct cultural proof of identity. Almost all systems use polymerase chain reaction. Real-time polymerase chain reaction is based on the polymerase chain reaction and routinely used to amplify and simultaneously quantify a targeted DNA molecule. Fluorescent dyes intercalating with double-stranded DNA and modified DNA oligonucleotide probes fluorescing when hybridized with a complementary DNA are used for real time quantification of the amplicons. The process of DNA quantification has demands on robust instrument and on high cost chemicals. Electrochemical detection is low cost and sensitive alternative method to detect nucleic acids. The main aim of this work is to propose novel electrochemical detector for monitoring PCR.

Klíčová slova

Viral infections, double-stranded DNA, oligonucleotide probes, fluorescence, PCR

Autoři

ADAM,V.; HÚSKA,D.; KŘÍŽKOVÁ,S.;HUBÁLEK,J.;KIZEK,R.

Rok RIV

2009

Vydáno

4. 7. 2009

Nakladatel

FEBS JOURNAL

Místo

Prague, CZECH REPUBLIC

ISSN

1742-464X

Periodikum

FEBS Journal

Ročník

276

Číslo

Suppl. 1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

95

Strany do

96

Strany počet

2

BibTex

@article{BUT93578,
  author="Vojtěch {Adam} and Dalibor {Húska} and Soňa {Křížková} and Jaromír {Hubálek} and René {Kizek}",
  title="Real-time polymerase chain reaction with electrochemical detection for detection pandemic viruses",
  journal="FEBS Journal",
  year="2009",
  volume="276",
  number="Suppl. 1",
  pages="95--96",
  issn="1742-464X"
}