Detail publikace

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

BABULA, P. VODIČKA, O. ADAM, V. KUMMEROVÁ, M. HAVEL, L. HOŠEK, J. PROVAZNÍK, I. ŠKUTKOVÁ, H. BEKLOVÁ, M. KIZEK, R.

Originální název

Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Polycyclic aromatic hydrocarbons (PAHs) belong to the group of the most important pollutants of the living environment, which are present in air, soils, freshwater, seawater and sediments. They have very substantial effects on all living organisms including plants and animals. Plants represent important point in PAHs food chain entry. Despite the fact that PAHs influence on animals is intensively studied, effect on plants is almost unknown. In our study, action of polycyclic aromatic hydrocarbon fluoranthene on a plant cell experimental model – tobacco BY-2 cell suspension culture – was studied. BY-2 cells were exposed to the fluoranthene in concentration range from 0 to 1000uM, duration of treatment was 120 h, respectively 5 days. Samples were collected in the strictly defined time intervals of 24 h. Exposure of the BY-2 cells led to significant changes in viability, changes in autofluorescence due to accumulation of fluoranthene in lipophilic cell compartments, especially biomembranes, and production of reactive oxygen species, which resulted in damage of biomembranes and disruption of their semipermeability and initiation of process of programmed cell death. Obtained results bring new knowledge about phytotoxicity of fluoranthene and mechanism of its action.

Klíčová slova

Fluoranthene, BY-2 cells, Viability, Programmed cell death, Fluoranthene accumulation

Autoři

BABULA, P.; VODIČKA, O.; ADAM, V.; KUMMEROVÁ, M.; HAVEL, L.; HOŠEK, J.; PROVAZNÍK, I.; ŠKUTKOVÁ, H.; BEKLOVÁ, M.; KIZEK, R.

Rok RIV

2012

Vydáno

21. 1. 2012

ISSN

0098-8472

Periodikum

ENVIRONMENTAL AND EXPERIMENTAL BOTANY

Ročník

2012

Číslo

78

Stát

Spojené království Velké Británie a Severního Irska

Strany od

117

Strany do

126

Strany počet

14

BibTex

@article{BUT88856,
  author="Petr {Babula} and Ondřej {Vodička} and Vojtěch {Adam} and Marie {Kummerová} and Ladislav {Havel} and Jan {Hošek} and Valentine {Provazník} and Helena {Vítková} and Miroslava {Beklová} and René {Kizek}",
  title="Effect of fluoranthene on plant cell model: Tobacco BY-2 suspension culture",
  journal="ENVIRONMENTAL AND EXPERIMENTAL BOTANY",
  year="2012",
  volume="2012",
  number="78",
  pages="117--126",
  issn="0098-8472"
}