Detail publikace

Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots

Originální název

Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots

Anglický název

Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots

Jazyk

en

Originální abstrakt

Luminescent quantum dots (QDs) have gained a great attention as promising candidates in optical detection of various biomolecules such as DNA, proteins, etc. In this paper, we focused on fabrication of QDs in the deposited form, which still belongs to less clarified and used approach in biosensing compared to colloidal one. The deposited QDs from non-toxic titanium dioxide (TiO2) were prepared by template based technique, namely anodic oxidation of titanium film through nanoporous alumina template. This method allows to creation of QDs strongly fixed on silicon substrate without addition of any other fixation matrix (e.g. polymer). Highly ordered TiO2 QDs with broad luminescence peak in visible range were achieved. The QDs array was characterized by SEM confirming the QDs size being below 10 nm, which is necessary to reach the quantum size effect. Preliminary results showed that subsequent modification of QDs surface with chicken DNA and bovine serum albumin (BSA) resulted in partial or complete quenching of fluorescence depending on the biomolecules concentration.

Anglický abstrakt

Luminescent quantum dots (QDs) have gained a great attention as promising candidates in optical detection of various biomolecules such as DNA, proteins, etc. In this paper, we focused on fabrication of QDs in the deposited form, which still belongs to less clarified and used approach in biosensing compared to colloidal one. The deposited QDs from non-toxic titanium dioxide (TiO2) were prepared by template based technique, namely anodic oxidation of titanium film through nanoporous alumina template. This method allows to creation of QDs strongly fixed on silicon substrate without addition of any other fixation matrix (e.g. polymer). Highly ordered TiO2 QDs with broad luminescence peak in visible range were achieved. The QDs array was characterized by SEM confirming the QDs size being below 10 nm, which is necessary to reach the quantum size effect. Preliminary results showed that subsequent modification of QDs surface with chicken DNA and bovine serum albumin (BSA) resulted in partial or complete quenching of fluorescence depending on the biomolecules concentration.

BibTex


@article{BUT75689,
  author="Jana {Drbohlavová} and Jana {Pekárková} and Radim {Hrdý} and Jan {Prášek} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek} and Tereza {Venerová} and Jaromír {Hubálek}",
  title="Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots",
  annote="Luminescent quantum dots (QDs) have gained a great attention as promising candidates in optical detection of various biomolecules such as DNA, proteins, etc. In this paper, we focused on fabrication of QDs in the deposited form, which still belongs to less clarified and used approach in biosensing compared to colloidal one. The deposited QDs from non-toxic titanium dioxide (TiO2) were prepared by template based technique, namely anodic oxidation of titanium film through nanoporous alumina template. This method allows to creation of QDs strongly fixed on silicon substrate without addition of any other fixation matrix (e.g. polymer). Highly ordered TiO2 QDs with broad luminescence peak in visible range were achieved. The QDs array was characterized by SEM confirming the QDs size being below 10 nm, which is necessary to reach the quantum size effect. Preliminary results showed that subsequent modification of QDs surface with chicken DNA and bovine serum albumin (BSA) resulted in partial or complete quenching of fluorescence depending on the biomolecules concentration.",
  address="ESG",
  chapter="75689",
  institution="ESG",
  number="2",
  volume="7",
  year="2012",
  month="february",
  pages="1424--1432",
  publisher="ESG",
  type="journal article in Web of Science"
}