Detail publikace

Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots

VACULOVIČOVÁ, M. PEKÁRKOVÁ, J. JANŮ, L. DRBOHLAVOVÁ, J. ADAM, V. HUBÁLEK, J. KIZEK, R.

Originální název

Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, biotin-conjugated glutathione was synthesized using peptide bonding of the biotin carboxy group and amino group of the g-glutamic acid to prepare an alternative coating for CdTe quantum dots (QDs). This type of coating combines the functionality of the biotin with the fluorescent properties of the QDs to create a specific, high-affinity fluorescent probe able to react with avidin, streptavidin and/or neutravidin. Biotin-functionalized glutathione-coated CdTe QDs were prepared by a simple one-step method using Na(2)TeO(3) and CdCl(2). Obtained QDs were separated from the excess of the biotin-conjugated glutathione by CE employing 300mM borate buffer with pH 7.8 as a background electrolyte. The detection of sample components was performed by the photometric detection at 214 nm and LIF employing Ar(+) ion laser (488 nm).

Klíčová slova

Biotin-conjugated glutathione, CE, Glutathione, Quantum dot, Streptavidin– biotin

Autoři

VACULOVIČOVÁ, M.; PEKÁRKOVÁ, J.; JANŮ, L.; DRBOHLAVOVÁ, J.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.

Rok RIV

2011

Vydáno

24. 5. 2011

Nakladatel

Wiley

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

32

Číslo

13

Stát

Spolková republika Německo

Strany od

1619

Strany do

1622

Strany počet

4

BibTex

@article{BUT75320,
  author="Markéta {Vaculovičová} and Jana {Pekárková} and Libor {Janů} and Jana {Drbohlavová} and Vojtěch {Adam} and Jaromír {Hubálek} and René {Kizek}",
  title="Biotin-modified glutathione as functionalized coating for bioconjugation of CdTe-based quantum dots",
  journal="Electrophoresis",
  year="2011",
  volume="32",
  number="13",
  pages="1619--1622",
  issn="0173-0835"
}