Detail publikace

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

ZÍTKA, O. KŘÍŽKOVÁ, S. KREJČOVÁ, L. HYNEK, D. GUMULEC, J. MASAŘÍK, M. SOCHOR, J. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. KIZEK, R.

Originální název

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

Anglický název

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients

Jazyk

en

Originální abstrakt

A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.

Anglický abstrakt

A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.

Dokumenty

BibTex


@article{BUT75097,
  author="Ondřej {Zítka} and Soňa {Křížková} and Ludmila {Krejčová} and David {Hynek} and Jaromír {Gumulec} and Michal {Masařík} and Jiří {Sochor} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}",
  title="Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients",
  annote="A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a
result of oxidation stress in prostate cancer patients.",
  address="Wiley",
  chapter="75097",
  doi="10.1002/elps.201100430",
  institution="Wiley",
  number="1",
  volume="2011 (32)",
  year="2011",
  month="september",
  pages="3207--3220",
  publisher="Wiley",
  type="journal article in Web of Science"
}