Detail publikace

Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker

KŘÍŽKOVÁ, S. RYVOLOVÁ, M. GUMULEC, J. MASAŘÍK, M. ADAM, V. MAJZLÍK, P. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Originální název

Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Prostate-specific antigen (PSA) is a routinely used marker of prostate cancer; however, the cut-off values for unambiguous positive/negative prostate cancer diagnoses are not defined. Therefore, despite the best effort, certain percentage of misdiagnosed cases is being recorded every year. For this reason, search formore specific diagnosticmarkers is of great interest. In this study, systematic comparison of PSA and metallothionein (MT) levels in blood serum of 46 prostate cancer-diagnosed patients is presented. It is clearly demonstrated that PSA levels vary significantly and despite normal total PSA values in the range of 0 - 4 ng/mL were obtained in over 36.9% of cases, positive prostate cancer was diagnosed by biopsy. In contrary, MT levels were considerably elevated in all tested samples and no significant variations were observed. These results are indicating the potential of MT as an additional prostate cancer marker reducing, in combination with PSA, the probability of false positive/negative diagnosis. To increase the throughput of the screening, chip-based capillary electrophoresis was suggested as a rapid and effective method for the fingerprinting analysis of prostate cancer from diseased blood sera.

Klíčová slova

Blood marker, Glutathione, Metallothionein, Prostate cancer, Prostate-specific antigen

Autoři

KŘÍŽKOVÁ, S.; RYVOLOVÁ, M.; GUMULEC, J.; MASAŘÍK, M.; ADAM, V.; MAJZLÍK, P.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

Rok RIV

2011

Vydáno

25. 7. 2011

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

2011

Číslo

32

Stát

Spolková republika Německo

Strany od

1952

Strany do

1961

Strany počet

10

BibTex

@article{BUT72631,
  author="Soňa {Křížková} and Markéta {Vaculovičová} and Jaromír {Gumulec} and Michal {Masařík} and Vojtěch {Adam} and Petr {Majzlík} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}",
  title="Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker",
  journal="Electrophoresis",
  year="2011",
  volume="2011",
  number="32",
  pages="1952--1961",
  issn="0173-0835"
}