Detail publikace

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

HÚSKA, D. FABRIK, I. BALOUN, J. ADAM, V. MASAŘÍK, M. HUBÁLEK, J. VAŠKŮ, A. TRNKOVÁ, L. HORNA, A. ZEMAN, L. KIZEK, R.

Originální název

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

Typ

článek v časopise - ostatní, Jost

Jazyk

angličtina

Originální abstrakt

Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs' resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.

Klíčová slova

Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance

Autoři

HÚSKA, D.; FABRIK, I.; BALOUN, J.; ADAM, V.; MASAŘÍK, M.; HUBÁLEK, J.; VAŠKŮ, A.; TRNKOVÁ, L.; HORNA, A.; ZEMAN, L.; KIZEK, R.

Rok RIV

2009

Vydáno

15. 3. 2009

Nakladatel

MDPI

Místo

MATTHAEUSSTRASSE 11, CH-4057 BASEL, SWITZERLAND

ISSN

1424-8220

Periodikum

SENSORS

Ročník

2009 (9)

Číslo

3

Stát

Švýcarská konfederace

Strany od

1355

Strany do

1369

Strany počet

15

URL

http://www.mdpi.com/1424-8220/9/3/1355

Plný text v Digitální knihovně

http://hdl.handle.net/11012/70083

BibTex

@article{BUT48743,
  author="HÚSKA, D. and FABRIK, I. and BALOUN, J. and ADAM, V. and MASAŘÍK, M. and HUBÁLEK, J. and VAŠKŮ, A. and TRNKOVÁ, L. and HORNA, A. and ZEMAN, L. and KIZEK, R.",
  title="Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance",
  journal="SENSORS",
  year="2009",
  volume="2009 (9)",
  number="3",
  pages="1355--1369",
  doi="10.3390/s90301355",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/9/3/1355"
}