Detail publikace

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Originální název

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Anglický název

Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy

Jazyk

en

Originální abstrakt

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

Anglický abstrakt

Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment. We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.

BibTex


@inproceedings{BUT36565,
  author="Tomáš {Zikmund} and Lukáš {Kvasnica} and Luděk {Lovicar} and Hana {Uhlířová} and Radim {Chmelík}",
  title="Dynamical Deformation Compensation of Phase in Digital Holographic Microscopy",
  annote="Digital holographic microscopy (DHM) is a powerful tool for observation of biological samples. The main advantage of DHM imaging is the possibility to reconstruct the intensity image and the phase image of an observed sample in real-time. The background of phase images is deformed and this deformation is changing during a long-term experiment.
We describe here a novel procedure of deformation compensation for sequence of phase images. This novel procedure is based on deformation compensation procedure for a single phase image which uses the least square method. The novel procedure is applicable to compensate deformation during observation in real-time. The proposed procedure is applied to phase images of cells obtained by a transmitted-light digital holographic microscope.",
  address="SPIE",
  booktitle="Proceedings of SPIE, Volume 7746",
  chapter="36565",
  howpublished="print",
  institution="SPIE",
  year="2010",
  month="december",
  pages="77460K-1--77460K-7",
  publisher="SPIE",
  type="conference paper"
}