Detail publikace

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

VIČAR, T. BALVAN, J. JAROŠ, J. JUG, F. KOLÁŘ, R. MASAŘÍK, M. GUMULEC, J.

Originální název

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Because of its non-destructive nature, label-free imaging is an important strategy for studying biological processes. However, routine microscopic techniques like phase contrast or DIC suffer from shadow-cast artifacts making automatic segmentation challenging. The aim of this study was to compare the segmentation efficacy of published steps of segmentation work-flow (image reconstruction, foreground segmentation, cell detection (seed-point extraction) and cell (instance) segmentation) on a dataset of the same cells from multiple contrast microscopic modalities.

Klíčová slova

Microscopy, Cell segmentation, Image reconstruction, Methods comparison, Differential contrast image, Quantitative phase imaging, Laplacian of Gaussians

Autoři

VIČAR, T.; BALVAN, J.; JAROŠ, J.; JUG, F.; KOLÁŘ, R.; MASAŘÍK, M.; GUMULEC, J.

Vydáno

28. 7. 2019

Nakladatel

BMC

Místo

London, England

ISSN

1471-2105

Periodikum

BMC BIOINFORMATICS

Ročník

20

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

25

Strany počet

25

URL

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2880-8

Plný text v Digitální knihovně

http://hdl.handle.net/11012/195836

BibTex

@article{BUT158175,
  author="Tomáš {Vičar} and Jan {Balvan} and Josef {Jaroš} and Florian {Jug} and Radim {Kolář} and Michal {Masařík} and Jaromír {Gumulec}",
  title="Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison",
  journal="BMC BIOINFORMATICS",
  year="2019",
  volume="20",
  number="1",
  pages="1--25",
  doi="10.1186/s12859-019-2880-8",
  issn="1471-2105",
  url="https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2880-8"
}