Detail publikace

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

TOLDE, O. GANDALOVIČOVÁ, A. KŘÍŽOVÁ, A. VESELÝ, P. CHMELÍK, R. RÖSEL, D. BRÁBEK, J.

Originální název

Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Observation and analysis of cancer cell behaviour in 3D environment is essential for full understanding of the mechanisms of cancer cell invasion. However, label-free imaging of live cells in 3D conditions is optically more challenging than in 2D. Quantitative phase imaging provided by coherence controlled holographic microscopy produces images with enhanced information compared to ordinary light microscopy and, due to inherent coherence gate effect, enables observation of live cancer cells’ activity even in scattering milieu such as the 3D collagen matrix. Exploiting the dynamic phase differences method, we for the first time describe dynamics of differences in cell mass distribution in 3D migrating mesenchymal and amoeboid cancer cells, and also demonstrate that certain features are shared by both invasion modes. We found that amoeboid fibrosarcoma cells’ membrane blebbing is enhanced upon constriction and is also occasionally present in mesenchymally invading cells around constricted nuclei. Further, we demonstrate that both leading protrusions and leading pseudopods of invading fibrosarcoma cells are defined by higher cell mass density. In addition, we directly document bundling of collagen fibres by protrusions of mesenchymal fibrosarcoma cells. Thus, such a non-invasive microscopy offers a novel insight into cellular events during 3D invasion.

Klíčová slova

Quantitative phase imaging , holographic microscopy, cancer cell invasion

Autoři

TOLDE, O.; GANDALOVIČOVÁ, A.; KŘÍŽOVÁ, A.; VESELÝ, P.; CHMELÍK, R.; RÖSEL, D.; BRÁBEK, J.

Vydáno

13. 8. 2018

Nakladatel

Springer Nature

ISSN

2045-2322

Periodikum

Scientific Reports

Číslo

8

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

13

Strany počet

13

URL

http://link.springer.com/article/10.1038/s41598-018-30408-7

Plný text v Digitální knihovně

http://hdl.handle.net/11012/84157

BibTex

@article{BUT149217,
  author="Ondřej {Tolde} and Aneta {Gandalovičová} and Aneta {Křížová} and Pavel {Veselý} and Radim {Chmelík} and Daniel {Rösel} and Jan {Brábek}",
  title="Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion",
  journal="Scientific Reports",
  year="2018",
  number="8",
  pages="1--13",
  doi="10.1038/s41598-018-30408-7",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-018-30408-7"
}