Detail publikace

Electrical Stimulation of ASAP1 with a Combination of Microelectrode Array and Fluorescent Techniques

SVOBODA, O. ČMIEL, V. BAIAZITOVA, L. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Originální název

Electrical Stimulation of ASAP1 with a Combination of Microelectrode Array and Fluorescent Techniques

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Optical noninvasive methods are modern tools in studying subcellular ion exchanges and corresponding membrane voltage alterations. In past decades, genetically encoded voltage indicators (GEVIs) received attention due to the low toxicity and enhanced detection limits. The very promising GEVI Accelerated Sensor of Action Potentials 1 (ASAP1) was expressed in HEK293 cells and extracellular stimulation was performed using a microelectrode-arrays integrated pulse generator. The results demonstrate high fluorescence response and changes, fast kinetics (1.5 ± 0.3 ms), and enhanced amplitude of fluorescence response, even if the voltage sensor is not completely repolarized. The results bring verification of advantageous properties of ASAP1 and confirm a big potential of modern voltage sensitive dyes and their application in neural activity sensing where these properties play an important role. At the same time, our testing method showed as a very advantageous in voltage sensitivity dyes properties validation.

Klíčová slova

ASAP1; fluorescence response; HEK293 cells; microelectrode array; transfection

Autoři

SVOBODA, O.; ČMIEL, V.; BAIAZITOVA, L.; PROVAZNÍK, I.; FOHLEROVÁ, Z.; HUBÁLEK, J.

Vydáno

23. 7. 2018

ISBN

978-1-5386-4695-3

Kniha

41st International Conference on Telecommunications and Signal Processing (TSP)

ISSN

1805-5435

Periodikum

International Conference on Telecommunications and Signal Processing (TSP)

Ročník

1805-5435

Číslo

41

Stát

Česká republika

Strany od

493

Strany do

496

Strany počet

4

URL

https://ieeexplore.ieee.org/document/8441414

BibTex

@inproceedings{BUT148916,
  author="Ondřej {Svoboda} and Vratislav {Čmiel} and Larisa {Chmelíková} and Valentine {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="Electrical Stimulation of ASAP1 with a Combination of Microelectrode Array and Fluorescent Techniques",
  booktitle="41st International Conference on Telecommunications and Signal Processing (TSP)",
  year="2018",
  journal="International Conference on Telecommunications and Signal Processing (TSP)",
  volume="1805-5435",
  number="41",
  pages="493--496",
  doi="10.1109/TSP.2018.8441414",
  isbn="978-1-5386-4695-3",
  issn="1805-5435",
  url="https://ieeexplore.ieee.org/document/8441414"
}