Detail publikace

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

ZUBAL, L. BONANI, W. MANIGLIO, D. CECCATO, R. RENČIUK, D. HAMPL, A. MIGLIARESI, C. JANČÁŘ, J. VOJTOVÁ, L.

Originální název

Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Dissolution and gelation procedures have a great influence on gelation time, microstructure and mechanical properties of reconstituted collagen products. We have investigated the dissolution of atelocollagen in CO2/water solutions at low temperature (4 degrees C) at different CO2 pressures (0.3-0.9 MPa), as well as gelation kinetics and physico-chemical properties of the hydrogel obtained after CO2 removal. Compared to conventional methods, the CO2-assisted technique resulted in faster soluble collagen dissolution and faster gelation into transparent gels characterized by thin 10 nm fibrils. Electrophoresis and CD spectroscopy demonstrated that the process did not denature the soluble collagen. The possibility to obtain collagen dissolution and gelation without the use of chemical agent other than water and CO2 makes this process particularly appealing for biomedical applications.

Klíčová slova

biocompatible polymers; collagen fibrillogenesis; structural analysis; viscoelastic properties; protein self-assembly

Autoři

ZUBAL, L.; BONANI, W.; MANIGLIO, D.; CECCATO, R.; RENČIUK, D.; HAMPL, A.; MIGLIARESI, C.; JANČÁŘ, J.; VOJTOVÁ, L.

Vydáno

1. 2. 2018

Nakladatel

Budapest University of Technology

ISSN

1788-618X

Periodikum

EXPRESS POLYM LETT

Ročník

12

Číslo

2

Stát

Maďarsko

Strany od

159

Strany do

170

Strany počet

12

URL

http://www.expresspolymlett.com/letolt.php?file=EPL-0008576&mi=cd

Plný text v Digitální knihovně

http://hdl.handle.net/11012/84160

BibTex

@article{BUT146700,
  author="Lukáš {Zubal} and Walter {Bonani} and Devid {Maniglio} and Riccardo {Ceccato} and Daniel {Renčiuk} and Aleš {Hampl} and Claudio {Migliaresi} and Josef {Jančář} and Lucy {Vojtová}",
  title="Soluble collagen dissolution and assembling in pressurized carbon dioxide water solutions",
  journal="EXPRESS POLYM LETT",
  year="2018",
  volume="12",
  number="2",
  pages="159--170",
  doi="10.3144/expresspolymlett.2018.14",
  issn="1788-618X",
  url="http://www.expresspolymlett.com/letolt.php?file=EPL-0008576&mi=cd"
}