Detail publikace

TERTIARY STRUCTURE PREDICTION OF POTENTIAL EFFLUX-PUMP PROTEIN IN CLOSTRIDIUM BEBERINCKII NRRL B-598

KOŠČOVÁ, P. SEDLÁŘ, K. KUPKOVÁ, K. KOLEK, J. PATÁKOVÁ, P. PROVAZNÍK, I.

Originální název

TERTIARY STRUCTURE PREDICTION OF POTENTIAL EFFLUX-PUMP PROTEIN IN CLOSTRIDIUM BEBERINCKII NRRL B-598

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

The fields of computational biochemistry and structural biology are growing rapidly. With various computational tools the whole workflow for in silico modeling of three-dimensional protein structures can be compiled. Here, we present a method for tertiary structure prediction for arcB1, a putative efflux pump gene, recently identified in the genomic sequence of Clostridium beijerinckii NRRL B-598. The strain C. beijerinckii NRRL B-598, formerly misidentified as C. pasteurianum, is an oxygen tolerant heterofermentative anaerobe with the ability of acetone-butanol fermentation, with butanol as the main product. Unfortunately, the mechanism of butanol efflux remains unknown. The translated AcrB1 protein sequence had a high similarity to the already described efflux-pump of Escherichia coli. To study these sequences in a structural context, we constructed a homology model for C. beijerinckii NRRL B-598 and compared it with the corresponding experimentally determined structure from E. coli. The predicted protein model helps us to understand its function and confirm that acrB1 is a gene for nonspecific efflux pump — a potential pump capable of butanol efflux.

Klíčová slova

Clostridium; ABE fermentation; efflux pump

Autoři

KOŠČOVÁ, P.; SEDLÁŘ, K.; KUPKOVÁ, K.; KOLEK, J.; PATÁKOVÁ, P.; PROVAZNÍK, I.

Vydáno

31. 10. 2017

Nakladatel

Ocean Design

Místo

Praha

ISBN

978-80-86238-62-3

Kniha

Proceedings of the 5th International Conference on Chemical Technology

Edice

1

Číslo edice

1

Strany od

26

Strany do

30

Strany počet

5

URL

http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf

BibTex

@inproceedings{BUT141676,
  author="Pavlína {Cicková} and Karel {Sedlář} and Kristýna {Kupková} and Jan {Kolek} and Petra {Patáková} and Valentine {Provazník}",
  title="TERTIARY STRUCTURE PREDICTION OF POTENTIAL EFFLUX-PUMP PROTEIN IN CLOSTRIDIUM BEBERINCKII NRRL B-598",
  booktitle="Proceedings of the 5th International Conference on Chemical Technology",
  year="2017",
  series="1",
  number="1",
  pages="26--30",
  publisher="Ocean Design",
  address="Praha",
  isbn="978-80-86238-62-3",
  url="http://www.icct.cz/AngiologyKlon-ICCT/media/system/ICCT2017-full_papers.pdf"
}