Detail publikace

All-in-one detector of circulating mRNA based on a smartphone

ČMIEL, V. GUMULEC, J. SVOBODA, O. RAUDENSKÁ, M. HUDCOVÁ, K. SEKORA, J. BALOGH, J. MASAŘÍK, M. PROVAZNÍK, I.

Originální název

All-in-one detector of circulating mRNA based on a smartphone

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Klíčová slova

mRNA, smartphone, PCR, cancer, metallothionein

Autoři

ČMIEL, V.; GUMULEC, J.; SVOBODA, O.; RAUDENSKÁ, M.; HUDCOVÁ, K.; SEKORA, J.; BALOGH, J.; MASAŘÍK, M.; PROVAZNÍK, I.

Vydáno

4. 3. 2016

Nakladatel

SPIE

Místo

San Francisco

ISBN

9781628419498

Kniha

Proceedings of SPIE

Číslo edice

9715

Strany od

1

Strany do

6

Strany počet

6

URL

http://dx.doi.org/10.1117/12.2213253

BibTex

@inproceedings{BUT122889,
  author="Vratislav {Čmiel} and Jaromír {Gumulec} and Ondřej {Svoboda} and Martina {Raudenská} and Kristýna {Hudcová} and Jiří {Sekora} and Jaroslav {Balogh} and Michal {Masařík} and Valentine {Provazník}",
  title="All-in-one detector of circulating mRNA based on a smartphone",
  booktitle="Proceedings of SPIE",
  year="2016",
  number="9715",
  pages="1--6",
  publisher="SPIE",
  address="San Francisco",
  doi="10.1117/12.2213253",
  isbn="9781628419498",
  url="http://dx.doi.org/10.1117/12.2213253"
}