Detail publikace

All-in-one detector of circulating mRNA based on a smartphone

Originální název

All-in-one detector of circulating mRNA based on a smartphone

Anglický název

All-in-one detector of circulating mRNA based on a smartphone

Jazyk

en

Originální abstrakt

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Anglický abstrakt

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

BibTex


@inproceedings{BUT122889,
  author="Vratislav {Čmiel} and Jaromír {Gumulec} and Ondřej {Svoboda} and Martina {Raudenská} and Kristýna {Hudcová} and Jiří {Sekora} and Jaroslav {Balogh} and Michal {Masařík} and Ivo {Provazník}",
  title="All-in-one detector of circulating mRNA based on a smartphone",
  annote="Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.",
  address="SPIE",
  booktitle="Proceedings of SPIE",
  chapter="122889",
  doi="10.1117/12.2213253",
  howpublished="online",
  institution="SPIE",
  year="2016",
  month="march",
  pages="1--6",
  publisher="SPIE",
  type="conference paper"
}