Detail publikace

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

BAIAZITOVA, L. SVOBODA, O. ČMIEL, V. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.

Originální název

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

Český název

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

Anglický název

The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency

Typ

článek ve sborníku

Jazyk

en

Originální abstrakt

In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.

Český abstrakt

In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.

Anglický abstrakt

In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.

Klíčová slova

HEK293 cells, transfection, channelrhodopsin-2, polyethylenimine, MATRA-A reagent

Rok RIV

2015

Vydáno

26.05.2015

Nakladatel

Masarykova Univerzita - MUNI PRESS

Místo

Brno, Czech Republic

ISBN

978-80-210-7857-4

Kniha

XV. Workshop of Physical Chemists and Electrochemists

Edice

1

Číslo edice

1

Strany od

71

Strany do

73

Strany počet

3

BibTex


@inproceedings{BUT115179,
  author="Larisa {Baiazitova} and Ondřej {Svoboda} and Vratislav {Čmiel} and Ivo {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency",
  annote="In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.",
  address="Masarykova Univerzita - MUNI PRESS",
  booktitle="XV. Workshop of Physical Chemists and Electrochemists",
  chapter="115179",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Masarykova Univerzita - MUNI PRESS",
  year="2015",
  month="may",
  pages="71--73",
  publisher="Masarykova Univerzita - MUNI PRESS",
  type="conference paper"
}