Detail publikace

Efekt kryopreservace na proudovou odezvu kanálu CaV 3.1 transfekovaného do buněk HEK293

SVOBODA, O. FOHLEROVÁ, Z. HUBÁLEK, J.

Originální název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Český název

Efekt kryopreservace na proudovou odezvu kanálu CaV 3.1 transfekovaného do buněk HEK293

Anglický název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Typ

abstrakt

Jazyk

en

Originální abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

Český abstrakt

Hlavní nevýhodou dlouhodobého uchovávání živých buněk v tekutém dusíku je jeho relativně rychlé odpařování. Z tohoto důvodu je nutné poměrně časté doplňování Dewarových nádob. V této práci byl použit nízkoteplotní mrazák jako ekonomičtější varianta k uchovávání v tekutém dusíku a byl studován vliv na proudovou odezvu z buněk HEK293 transfekovaných kanálem CaV 3.1. Ze získaných výsledků je zřejmé, že mezi proudovými odezvami z buněk zchovávaných osm měsíců v nízkoteplotním mrazáku a buňkami uchovávanými v tekutém dusíku není žádná signifikatní změna. Uchovávání buněk v nízkoteplotním mrazáku tak může být jednou z alternativ k tekutému dusíku.

Anglický abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

Vydáno

23.04.2015

Nakladatel

Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních

Místo

Brno

ISBN

9788021421451483

Kniha

Proceedings of the 21st Student Competition Conference

Edice

1

Číslo edice

1

Strany od

491

Strany do

494

Strany počet

4

BibTex


@misc{BUT114476,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells",
  annote="The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively
fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments,
we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen
and we would like to show that there is no significant influence of long-term storage of mammalian
cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic
of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured
from cells stored for eight months at -80 °C and compared with previously published papers. The
current traces and I-V curves showed that there are no changes in current response between cells
frozen at a low temperature and previously published results. From our results can be concluded
that the low temperature freezer is an adequate option for storage of mammalian cells for a couple
of months.",
  address="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  booktitle="Proceedings of the 21st Student Competition Conference",
  chapter="114476",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  year="2015",
  month="april",
  pages="491--494",
  publisher="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  type="abstract"
}