Detail publikace

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Originální název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Anglický název

The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells

Jazyk

en

Originální abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

Anglický abstrakt

The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments, we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen and we would like to show that there is no significant influence of long-term storage of mammalian cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured from cells stored for eight months at -80 °C and compared with previously published papers. The current traces and I-V curves showed that there are no changes in current response between cells frozen at a low temperature and previously published results. From our results can be concluded that the low temperature freezer is an adequate option for storage of mammalian cells for a couple of months.

BibTex


@misc{BUT114476,
  author="Ondřej {Svoboda} and Zdenka {Fohlerová} and Jaromír {Hubálek}",
  title="The effect of cryopreservation on the current response of CaV 3.1 transfected HEK293 cells",
  annote="The main disadvantage of long-term storage of living cells in liquid nitrogen is its relatively
fast evaporation. For this reason, it is necessary to refill Dewar flask quite often. In our experiments,
we used low temperature freezer (-80 °C) as more economical option to liquid nitrogen
and we would like to show that there is no significant influence of long-term storage of mammalian
cell-sat a low temperature. The effect of temperature was studied as the electrophysiological characteristic
of the whole-cell Ca2+ current in HEK293 cells. The current responses were measured
from cells stored for eight months at -80 °C and compared with previously published papers. The
current traces and I-V curves showed that there are no changes in current response between cells
frozen at a low temperature and previously published results. From our results can be concluded
that the low temperature freezer is an adequate option for storage of mammalian cells for a couple
of months.",
  address="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  booktitle="Proceedings of the 21st Student Competition Conference",
  chapter="114476",
  edition="1",
  howpublished="electronic, physical medium",
  institution="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  year="2015",
  month="april",
  pages="491--494",
  publisher="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  type="abstract"
}