Detail publikace

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

Originální název

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

Anglický název

Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis

Jazyk

en

Originální abstrakt

In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.

Anglický abstrakt

In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.

BibTex


@misc{BUT105322,
  author="Jiří {Kukačka} and Dalibor {Húska} and Milan {Pouch} and Soňa {Křížková} and Jana {Drbohlavová} and Jana {Pekárková} and Vojtěch {Adam} and Jaromír {Hubálek} and Eva {Klapková} and Richard {Průša} and René {Kizek}",
  title="Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis",
  annote="In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25–35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPs was sufficient for effective isolation of the molecule of the interest.",
  address="FEDERATION AMER SOC EXP BIOL",
  chapter="105322",
  institution="FEDERATION AMER SOC EXP BIOL",
  year="2011",
  month="april",
  publisher="FEDERATION AMER SOC EXP BIOL",
  type="abstract"
}