Detail publikace

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Originální název

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Anglický název

Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs

Jazyk

en

Originální abstrakt

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.

Anglický abstrakt

In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.

BibTex


@article{BUT100954,
  author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}",
  title="Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs",
  annote="In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml  1 of ODN-SH-Cd.",
  chapter="100954",
  doi="10.1007/s10337-012-2327-0",
  number="7-8",
  volume="76",
  year="2013",
  month="april",
  pages="355--362",
  type="journal article in Web of Science"
}