Detail projektu

Directed evolution of polyhydroxyalkanoates producing bacteria

Období řešení: 01.01.2019 — 31.12.2021

O projektu

The main goal of the project is to perform the evolutionary engineering of PHAs producing bacteria in order to better understand adaptive mechanisms of industrially relevant bacteria and to explore the role of PHAs in their adaptation to adverse conditions. Further, our aim is also to obtain robust bacterial strains resistant to the most important stress factors associated with anthropogenic and/or environmental stressors for in situ bioremediation as well as PHA producers adapted to microbial inhibitors present in lignocellulose-based substrates. To reach these goals, highly multidisciplinary approach combining various fields of science such as biochemistry, molecular biology, biophysics and also advanced techniques of microspectroscopy and cell manipulation will be applied.

Klíčová slova
evolutionary engineering; PHA; bacteria; stress response; adaptation

Označení

GA19-20697S

Originální jazyk

angličtina

Řešitelé

Obruča Stanislav, doc. Ing., Ph.D.
- hlavní řešitel (01.01.2019 - 31.12.2020)
Sedláček Petr, Ing., Ph.D.
- spoluřešitel (01.01.2019 - 31.12.2020)

Útvary

Centrum materiálového výzkumu
- příjemce (03.04.2018 - nezadáno)

Zdroje financování

Grantová agentura České republiky - Standardní projekty
- plně financující (2019-01-01 - 2021-12-31)

Výsledky

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; KOLLER, M.; KOVALČÍK, A.; OBRUČA, S. Adaptation of cupriavidus necator to levulinic acid for enhanced production of P(3HB- co -3HV) copolyesters. BIOCHEMICAL ENGINEERING JOURNAL, 2019, vol. 151, no. 11, p. 1-10. ISSN: 1369-703X.
Detail

PERNICOVÁ, I.; KUČERA, D.; NEBESÁŘOVÁ, J.; KALINA, M.; NOVÁČKOVÁ, I.; KOLLER, M.; OBRUČA, S. Production of polyhydroxyalkanoates on waste frying oil employing selected Halomonas strains. BIORESOURCE TECHNOLOGY, 2019, vol. 292, no. 11, p. 1-4. ISSN: 0960-8524.
Detail

KUČERA, D.; NOVÁČKOVÁ, I.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S. Biotechnological Production of Poly(3-Hydroxybutyrate-co-4-Hydroxybutyrate-co-3-Hydroxyvalerate) Terpolymer by Cupriavidus sp. DSM 19379. Bioengineering, 2019, vol. 6, no. 3, p. 74-84. ISSN: 2306-5354.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; KUČERA, D.; OBRUČA, S. Isolation extremopilic bacteria producing polyhydroxyalkanoate from natural sample. The biomania student scientific meeting and eusynbios symposium 2019 Book of abstracts. Brno, Czech Republic: Masaryk University Press, 2019. p. 114-114. ISBN: 978-80-210-9373-7.
Detail

OBRUČA, S.; SEDLÁČEK, P.; SLANINOVÁ, E.; FRITZ, I.; DAFFERT, C.; MEIXNER, K.; ŠEDRLOVÁ, Z.; KOLLER, M. Novel unexpected functions of PHA granules. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, vol. 104, no. 11, p. 4795-4810. ISSN: 0175-7598.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; OBRUČA, S.; SEDLÁČEK, P.; POŘÍZKA, J.; PERNICOVÁ, I. Adaptation of PHA producing bacteria Cupriavidus necator H16 and Halomonas halophila to biotechnologically relevant stressors. 10th European Symposium on Biopolymers - Program. 2019. p. 70-70.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S. Application of evolutionary engineering for enhancement of robustness and producing capacity of selected microbial PHA producers. The Biomania student scientific meeting and Eusynbios symposium 2019 - Book of Abstracts. 1. 2019. p. 120-120. ISBN: 978-80-210-9373-7.
Detail

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; SEDLÁČEK, P.; PERNICOVÁ, I.; OBRUČA, S. Evolutionary engineering approach for enhancement of growth characteristics and producing capacity of selected PHA producing microorganisms. Studentská odborná konference Chemie je život 2019 - Sborník abstraktů. Brno: Vysoké učení technické v Brně, Fakulta chemická, 2019. p. 58-58. ISBN: 978-80-214-5807-9.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; SEDLÁČEK, P.; KOUŘILOVÁ, X.; KOLLER, M.; OBRUČA, S. Application of osmotic challenge for enrichment of microbial consortia in polyhydroxyalkanoates producing thermophilic and thermotolerant bacteria and their subsequent isolation. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, vol. 144, no. 2, p. 698-704. ISSN: 0141-8130.
Detail

PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; HERNÁNDEZ, N.; PRIETO, M.A.; OBRUČA, S. Thermophilic bacteria isolated from compost as producers of polyhydroxyalkanoates. 10th European Symposium on Biopolymers. Straubing, Germany: C.A.R.M.E.N.e.V., 2019. p. 72-72.
Detail