Detail předmětu

Advanced fluorescence techniques

FCH-DA_PFTAk. rok: 2020/2021

The course is designed as a practically focused seminar, which will enable students to acquaint themselves with advanced fluorescence techniques on a suitable model system. Theoretical and practical lessons will alternate within the seminar. The theoretical parts will focus on specific aspects of preparation, measurement and interpretation of the fluorescence techniques and will build on the self-study of the fundamental fundamentals of the given techniques. The practical part includes the preparation of the model system itself (associative colloid system), measurement and evaluation. Within the subject, the following techniques will be discussed: time-resolved emission spectroscopy, anisotropy, resonance energy transfer, fluorescence quenching, fluorescence correlation spectroscopy, two-color fluorescence cross-correlation spectroscopy. Together with the introductory lesson of these 6 themes with the theoretical and practical part will create the framework of the semester. At the end of the semester, the basics will be created to create a short monograph on the behavior of the selected system from the perspective of advanced fluorescence techniques.

Výsledky učení předmětu

Výstupem studia jsou praktické dovednosti v pokročilých experimentálních metodách fluorescenční spektroskopie. Studenti se seznámí se základy technik používaných v pokročilém výzkumu, naučí se základy interpretace takovýchto dat a na jednoduchém systému si tyto znalosti ověří.

Prerekvizity

Předpokládájí se znalosti z oboru optických spektroskopií a základní znalosti o jevu fluorescence.

Doporučená nebo povinná literatura

Lakowicz, J.R. Principles of Fluorescence 3rd ed., Springer, Baltimore, 2006 (EN)
Valeur, B.; Berberan-Santos, M.N. Molecular Fluorescence: Principles and Applications, Wiley‐VCH Verlag GmbH & Co. KGaA, 2012 (EN)

Plánované vzdělávací činnosti a výukové metody

Předmět je vyučován jako soubor teoretických a praktických seminářů.

Způsob a kritéria hodnocení

Study results are verified pursuant to Article 34, Paragraph 7 of Study and Examination Rules of BUT. Students are given a credit for submitting protocols for each technique they studied. Protocols are processed in the following format: principles of the technique, the aim and exact methods used for measurement, collected data, their evaluation and interpretation, assessment of the impact of the technique on biophysical practice.

Jazyk výuky

angličtina

Osnovy výuky

The course is designed as a practically focused seminar, which will enable students to acquaint themselves with advanced fluorescence techniques on a suitable model system. Theoretical and practical lessons will alternate within the seminar. The theoretical parts will focus on specific aspects of preparation, measurement and interpretation of the fluorescence techniques and will build on the self-study of the fundamental fundamentals of the given techniques. The practical part includes the preparation of the model system itself (associative colloid system), measurement and evaluation. Within the subject, the following techniques will be discussed: time-resolved emission spectroscopy, anisotropy, resonance energy transfer, fluorescence quenching, fluorescence correlation spectroscopy, two-color fluorescence cross-correlation spectroscopy. Together with the introductory lesson of these 6 themes with the theoretical and practical part will create the framework of the semester. At the end of the semester, the basics will be created to create a short monograph on the behavior of the selected system from the perspective of advanced fluorescence techniques.
1) Time resolved emission spectroscopy
2) Time resolved emission spectroscopy - practice
3) Anisotropy
4) Anisotropy - Practicum
5) Resonant energy transfer
6) Resonant energy transfer - practice
7) Fluorescence quenching
8) Fluorescence quenching - practice
9) Fluorescence correlation spectroscopy
10 Fluorescence Correlation Spectroscopy - Practice
11) Two-color fluorescence cross-correlation spectroscopy
12) Two-color fluorescence cross-correlation spectroscopy - practice
13) Presentation of the results of individual techniques

Cíl

The aim of this course is to introduce and use practical examples of fluorescence techniques that can be used in various disciplines ranging from material sciences to biochemical applications.

Vymezení kontrolované výuky a způsob jejího provádění a formy nahrazování zameškané výuky

Předpokládájí se znalosti z oboru optických spektroskopií a základní znalosti o jevu fluorescence.

Zařazení předmětu ve studijních plánech

  • Program DPAP_BCH doktorský, 1. ročník, zimní semestr, 0 kreditů, povinně volitelný
  • Program DKAP_BCH doktorský, 1. ročník, zimní semestr, 0 kreditů, povinně volitelný