Course detail

Selected Topics in Electron Microscopy

FSI-TM0Acad. year: 2020/2021

The course deals with problems of electron microscopy and related methods. Accented are physical principles, construction and production of electron microscopes.

Learning outcomes of the course unit

Knowledge of the principles of construction of electron microscope and functionality of its parts.


Knowledge of electromagnetism on the level defined by the textbook HALLIDAY, D. - RESNICK, R. - WALKER, J.: Fundamentals of Physics. J. Wiley and Sons.


Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

GOLDSTEIN, Joseph I. Scanning electron microscopy and X-ray microanalysis. 3rd ed. New York: Kluwer Academic/Plenum publishers, 2003. xix, 689 s. ISBN 0-306-47292-9
REIMER, Ludwig. Transmission Electron Microscopy :Physics of Image Formation and Microanalysis. 3. ed. Berlin, 1993. 545 s. ISBN 3-540-56849-2
REIMER, Ludwig. Scanning electron microscopy :physics of image formation and microanalysis. Berlin, 1985. 457 s. ISBN 3-540-13530-8

Planned learning activities and teaching methods

lectures + hands on lesson

Assesment methods and criteria linked to learning outcomes

credits, based on final discussion

Language of instruction


Work placements

Not applicable.


After succesfull passing the course, the students should be able to:
- describe the construction of various types electron microscopes
- characterise individual physical phenomena yielding the image and discuss the function of the aparatus blocks
- evaluate the utility of different types EM for particular sample measurement.

Specification of controlled education, way of implementation and compensation for absences

Participation at lectures

Classification of course in study plans

  • Programme M2A-P Master's

    branch M-PMO , 1. year of study, summer semester, 2 credits, elective

  • Programme N-FIN-P Master's, 1. year of study, summer semester, 2 credits, elective

  • Programme M2A-P Master's

    branch M-PMO , 2. year of study, summer semester, 2 credits, elective

  • Programme N-FIN-P Master's, 2. year of study, summer semester, 2 credits, elective

Type of course unit



26 hours, optionally

Teacher / Lecturer


1. Basics of scanning and transmission electron microscopy
2. Applications of electron microscopy
3. Electron and ion optics
4. Sources of electrons and ions
5. Interaction of electrons and ions with solid state matter
6. Ssignal detection im electron microscopy
7. Vacuum system
8. Sample manipulation inside electron microscope
9. Sample stages
10. Electron microscope as a system
11. Contrast of image 1. (SEM)
12. Contrast of image 2. (TEM)
13. Electron microscope as an analytical lab
14. Mopdern trends in electron microscopy
15. Practical demonstration