FEKT-LBFYAcad. year: 2020/2021
Interpretation of bioelectric phenomena. Electrical activity of living tissue on molecular, cellular and organ level. Methods of measurement of membrane voltage and membrane currents in isolated cells, recording of random pulse signals from membrane channels on molecular level. Origin and propagation of impulses of action voltage. Cellular basis of diagnostically significant electromagnetic field generated by organs. Coupling between electrical excitation and muscle contraction. Introduction to biomechanics. Mechanics of cardiovascular system. Introduction to biothermodynamics. Gibbs energy and electrochemical potentials in biophysics. Biophysics of ecosystem.
Learning outcomes of the course unit
Having finished the subject, the student is able:
- applying the known physical laws to explain genesis of membrane voltage in the living cells and to define quantities that appear in the Nernst formula for equilibrium voltages,
- to describe electrical equivalent scheme of the cell,
- to explain origin of action voltages in excitable cells and mechanism of its propagation along cell fibers,
- to describe principles of the methods of measurement of membrane voltage and membrane current,
- to characterize electrical signals recorded on cellular and molecular level and to explain their mutual relations,
- to describe differences between the function of membrane channels and carriers,
- to describe the relation between the propagated excitation at the level of cell and genesis of electromagnetic field in the surrounding tissue,
- to describe origin of ECG signal as a result of action voltage propagation in the net of cardiac cells (syncytium),
- to prepare physiological solutions including measurement and adjustment of their pH, to measure tissue impedance and properties of the electrodes,
- to explain principles of excitation-contraction coupling in muscle cells,
- to apply physical principles to situation in cardiovascular system,
- to define the terms ‘chemical potential’ and ‘electrochemical potential’, and to illustrate their importance in interpretation of bioelectric phenomena,
- to discuss the function of the ecosystem (circulation of substance and flow of energy) from the viewpoint of thermodynamics.
The subject knowledge on the Bachelor´s degree level is requested.
Recommended optional programme components
Recommended or required reading
Biophysics - Wikipedie - the free encyklopedia
S. Silbernagl, A. Despopoulos: Atlas fyziologie člověka, GRADA Publishing, a.s. 2004 (CS)
F. Bezanilla: Electrophysiology and the Molecular Basis of Excitability. (University of California at Los Angeles) http://nerve.bsd.uchicago.edu/ (EN)
T.F. Weiss: Cellular Biophysics, Massachusetts Institute of Technology,1996 (vol.2 –ISBN 0-262-23184-0)) (EN)
R.Plonsey, R.C. Barr: Bioelectricity: A Quantitative Approach. Plenum Press, New York, 1988 (EN)
J. Šimurda: Bioelektrické jevy I, CERM Brno, 1995 (CS)
Planned learning activities and teaching methods
Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.
Assesment methods and criteria linked to learning outcomes
Requirements for completion of a course are specified by a regulation issued by the lecturer responsible for the course and updated for every.
Language of instruction
The aim is to teach students to apply physical theories and experimental methods to living organisms.
Specification of controlled education, way of implementation and compensation for absences
The content and forms of instruction in the evaluated course are specified by a regulation issued by the lecturer responsible for the course and updated for every academic year.