Course detail

Advanced fluorescence techniques

FCH-DC_PFTAcad. year: 2020/2021

Not applicable.

Learning outcomes of the course unit

Graduates of this course will understand the basic concepts of fluorescence spectroscopy. They will orientate themselves in stationary, time resolved and microscopic techniques. They will be provided with practical information on how to measure and analyze relevant data.


physics, physical chemistry


Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Lakowicz, J.R. Principles of Fluorescence 3rd ed., Springer, Baltimore, 2006 (EN)
Valeur, B.; Berberan-Santos, M.N. Molecular Fluorescence: Principles and Applications, Wiley‐VCH Verlag GmbH & Co. KGaA, 2012 (EN)

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Exam: written and oral.

Language of instruction


Work placements

Not applicable.

Course curriculum

- What is light and matter? Historical overview. Description tools.
- Scattering and absorption of radiation - electron, vibrational and rotational transitions. The fate of the excited state. Kinetics. Nonlinear optical phenomena.
- Solvatochromism and other environmental effects.
- Absorption spectrophotometry - uv-vis-ir, spectrophotometers and advanced techniques.
- Fluorescence spectroscopy stationary - fluorimeters, types of spectra, quantum yield
- Time-resolved fluorescence spectroscopy - time vs. time frequency domain, instrumentation and principles
- Microscopy - visible, fluorescent, confocal - principles and instrumentation
- Fluorescence correlation spectroscopy - autocorrelation function, cross-correlation function, instrumentation, measurement principles, models
- "Supermicroscopy" - STED, dSTORM, PALM, PAINT etc. - principles and applications
- The most common mistakes and errors in measurement.


Learning outcomes of the course unit The aim of the course is to present basic and advanced information about techniques using the fluorescence phenomenon.

Specification of controlled education, way of implementation and compensation for absences

Attendance to lectures is not required but is recommended.

Classification of course in study plans

  • Programme DPCP_BCH Doctoral, 1. year of study, winter semester, 0 credits, compulsory-optional
  • Programme DKCP_BCH Doctoral, 1. year of study, winter semester, 0 credits, compulsory-optional