Advanced fluorescence techniques
FCH-DC_PFTAcad. year: 2020/2021
Learning outcomes of the course unit
Graduates of this course will understand the basic concepts of fluorescence spectroscopy. They will orientate themselves in stationary, time resolved and microscopic techniques. They will be provided with practical information on how to measure and analyze relevant data.
physics, physical chemistry
Recommended optional programme components
Recommended or required reading
Lakowicz, J.R. Principles of Fluorescence 3rd ed., Springer, Baltimore, 2006 (EN)
Valeur, B.; Berberan-Santos, M.N. Molecular Fluorescence: Principles and Applications, Wiley‐VCH Verlag GmbH & Co. KGaA, 2012 (EN)
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Exam: written and oral.
Language of instruction
- What is light and matter? Historical overview. Description tools.
- Scattering and absorption of radiation - electron, vibrational and rotational transitions. The fate of the excited state. Kinetics. Nonlinear optical phenomena.
- Solvatochromism and other environmental effects.
- Absorption spectrophotometry - uv-vis-ir, spectrophotometers and advanced techniques.
- Fluorescence spectroscopy stationary - fluorimeters, types of spectra, quantum yield
- Time-resolved fluorescence spectroscopy - time vs. time frequency domain, instrumentation and principles
- Microscopy - visible, fluorescent, confocal - principles and instrumentation
- Fluorescence correlation spectroscopy - autocorrelation function, cross-correlation function, instrumentation, measurement principles, models
- "Supermicroscopy" - STED, dSTORM, PALM, PAINT etc. - principles and applications
- The most common mistakes and errors in measurement.
Learning outcomes of the course unit The aim of the course is to present basic and advanced information about techniques using the fluorescence phenomenon.
Specification of controlled education, way of implementation and compensation for absences
Attendance to lectures is not required but is recommended.