Course detail

Special molecular biotechnology

FCH-DC_SMBAcad. year: 2020/2021

Not applicable.

Language of instruction

Czech

Number of ECTS credits

0

Mode of study

Not applicable.

Guarantor

prof. Mgr. Václav Brázda, Ph.D.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Learning outcomes of the course unit

Not applicable.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Not applicable.

Course curriculum

Basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC. Identification of recombinant DNA. Gene expression in microbial (bacteria, yeast) and animal expression systems, markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection).

Work placements

Not applicable.

Aims

Not applicable.

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Glick B. R., Pasternak J. J.: Molecular Biotechnology. ASM Press, Washington 2003. (EN)
J. Čechová, J. Coufal, E.B. Jagelská, M. Fojta, V. Brázda, P73, Like Its P53 Homolog, Shows Preference for Inverted Repeats Forming Cruciforms, PLoS One. 13 (2018). doi:10.1371/journal.pone.0195835. (EN)
https://www.thermofisher.com/cz/en/home/life-science/cloning/gateway-cloning.html (EN)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme DPCP_BCH Doctoral, 1. year of study, winter semester, compulsory-optional
  • Programme DKCP_BCH Doctoral, 1. year of study, winter semester, compulsory-optional