Course detail

Molecular Biotechnology

FCH-MCO_MOBAcad. year: 2020/2021

The course is focused on the explanation of procedures used in molecular biotechnology for construction of genetically modified organisms with required characteristics. It explains the procedures used for gene transfer from one organism to the other with the aim to obtain an useful product including procedures used for optimalisation of gene expression and stability of recombinant proteins in procaryotic and eucaryotic cells. The impact of transgenic microorganisms, plants and animals in our life and their practical use are explained in addition to their construction.

Learning outcomes of the course unit

Theoretical aspects of various experimental techniques used in molecular biotechnology research in GMO construction and their practical use.

Prerequisites

Pass out the lectures of Molecular genetics I and II is recomended

Co-requisites

Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is written (test) and oral. The student had to proof the understanding of the subject.

Language of instruction

Czech

Work placements

Not applicable.

Course curriculum


924/5000
• The relationship between chemical production and biotechnology.
• DNA isolation and recombinant DNA technology.
• DNA cloning, polymerase chain reaction, PCR utilization in clinical and food diagnostics
• Cloning vectors and their use.
• Transformacfe - Transfer of foreign DNA into cells. Selection of cloned gene cells.
• Fusion proteins. Translational expression vectors. Enhancement of protein stability. Integration and shuttle vectors.
• Yeast expression systems. Systems using insect cells, mammalian expression systems.
• Biotechnological use of genetically modified micro-organisms.
• Transgenic plants and animal animals.
• Bioinformatics in gene biotechnology
• Use of bioinformatic approaches in the study of nucleic acids and proteins
• Gene therapy Diseases treatable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy.
• Development, characterization and antibody production

Aims

Lectures cover methodologies commonly used in molecular biotechnology in construction of transgenic organims and with the use of these organisms.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended

Classification of course in study plans

  • Programme AKREDITACE Master's

    specialization BCH , 1. year of study, winter semester, 4 credits, compulsory-optional

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1. year of study, winter semester, 4 credits, compulsory

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1. year of study, winter semester, 4 credits, compulsory

  • Programme AKREDITACE Master's

    specialization CHBL , 1. year of study, winter semester, 4 credits, compulsory-optional

  • Programme NPCP_MA Master's

    branch NPCO_MA , 1. year of study, winter semester, 4 credits, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1. year of study, winter semester, 4 credits, compulsory-optional

Type of course unit

 

Guided consultation in combined form of studies

26 hours, obligation not entered

Teacher / Lecturer

eLearning