Course detail

Bioanalytical Methods

FCH-MCO_BIMAcad. year: 2020/2021

Not applicable.

Learning outcomes of the course unit

Not applicable.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Recommended optional programme components

Not applicable.

Recommended or required reading

Záruba, Kamil. Analytická chemie. Praha: VSCHT v Praze, 2016. (CS)
Manz, A. Bioanalytical chemistry. London: Imperial College Press, 2015. (EN)
Ramesh, Vasudevan. Biomolecular and Bioanalytical Techniques. Wiley, 2019. (EN)

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is oral

Language of instruction

Czech

Work placements

Not applicable.

Course curriculum

1) Introduction to bioanalytical methods and their application
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques

Aims

The aim is to acquire knowledge about the methods that are used for analysis of biological materials.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended

Classification of course in study plans

  • Programme AKREDITACE Master's

    specialization BCH , 2. year of study, winter semester, 4 credits, compulsory-optional
    specialization CHPL , 2. year of study, winter semester, 4 credits, compulsory-optional
    specialization PCH , 2. year of study, winter semester, 4 credits, compulsory-optional

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 2. year of study, winter semester, 4 credits, compulsory-optional

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 2. year of study, winter semester, 4 credits, compulsory-optional

  • Programme AKREDITACE Master's

    specialization CHBL , 2. year of study, winter semester, 4 credits, compulsory-optional
    specialization BF , 2. year of study, winter semester, 4 credits, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1. year of study, winter semester, 4 credits, compulsory-optional

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

Guided consultation in combined form of studies

26 hours, obligation not entered

Teacher / Lecturer

eLearning