Course detail
Biochemistry I
FCH-BAA_BCH1Acad. year: 2019/2020
Organization and composition of living cells and cellular compartments. Amino acids - composition and structure, peptides, proteins. Methods of isolation and separation of proteins. Functional roles of proteins. Nucleic acids. Carbohydrates. Lipids. Biomembranes. Metabolic pathways. termodynamic relationships and energy-rich components. Enzymology, enzyme catalysis. Enzyme kinetics, inhibition of enzymes. Regulation of enzyme activity. Nucleic acids. Applied biochemistry.
Supervisor
Learning outcomes of the course unit
After passing of the course Biochemistry I is student mastered of overview about cell composition, structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, basic knowledge about structure of biomemrbanes and types of membrane transport are introduced and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, overview about methods of isolation and characterization of biopolymers will be obtained and some applications including enzyme engineering and genetic engineering will be discussed.
Prerequisites
Knowledge of organic chemistry, basic knowledge of physical chemistry.
Co-requisites
Not applicable.
Recommended optional programme components
Not applicable.
Recommended or required reading
Metzler D.A.: Biochemistry. Academic Press, 2003. (EN)
Berg, Tymoczko, Stryer: Biochemistry. W.H.Freeman, 2002. (EN)
Alberts et al: Molecular Biology of the Cell. Garland Science, 2003. (EN)
Elektronická verze přednášek. (EN)
Planned learning activities and teaching methods
The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Credit test. Examination - written and oral part.
Language of instruction
English
Work placements
Not applicable.
Course curriculum
Organization and composition of living cells and cellular compartments.
Amino acids - composition and structure, peptides, proteins.
Methods of isolation, purification and separation of proteins.
Functional roles of proteins.
Carbohydrates - monosaccharides, polysaccharides, glycoproteins.
Lipids - structure and classification. Triglycerides, phospholipids. Isoprenoids.
Biomembranes. Membrane transport.
Enzymology, enzyme catalysis. Substrate specificity. Cofactors. Regulation of enzyme activity.
Enzyme kinetics, Michaelis-Menten equation. Inhibition of enzymes.
Metabolic pathways. termodynamic relationships and energy-rich components.
Nucleic acids - structure and function. DNA, RNA.
Applide biochemistry - modern biotechnology, genetic engineering.
Aims
The aim of the course Biochemistry I is to introduce cell composition and to characterize structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, structure of biomemrbanes and types of membrane transport are explained and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, methods of isolation and characterization of biopolymers are introduced and some applications including enzyme engineering and genetic engineering are discussed.
Specification of controlled education, way of implementation and compensation for absences
none
Classification of course in study plans
- Programme BPCP_CHCHT_INT Bachelor's
branch BPCO_SCH , 1. year of study, summer semester, 4 credits, optional
- Programme NPCP_CHM_INT Master's
branch NPCO_CHM , 1. year of study, summer semester, 4 credits, optional