Course detail

Laboratory Technology for Genomics and Proteomics

FEKT-FLABAcad. year: 2018/2019

The course is focused on laboratory equipment used in genomics and proteomics. The study is oriented to knowledge on DNA and proteom's analysis methods.

Language of instruction

Czech

Number of ECTS credits

6

Mode of study

Not applicable.

Guarantor

doc. Ing. Jana Kolářová, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Learning outcomes of the course unit

The student is able to:
- specify the basic requirements for laboratory space,
- explain the principle of fluorescence of biological molecules, to name the most widely used fluorescent markers for DNA and proteins,
- explain the principle of DNA sequences, to describe structural design DNA sequencer,
- explain the principle of PCR and real-time PCR, to describe the structural design of PCR and real-time PCR,
- describe the chromatographic separation methods, describe the importance of the chromatogram and identify the separated substances
- explain the principle of liquid and gas chromatography,
- explain the principle of flow cytometry and describe the structural design of the flow cytometer
- explain the principles of the various types of spectroscopy and their application.

Prerequisites

The subject knowledge on the Bachelor's degree level is requested.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Teaching methods depend on the type of the course unit as specified in the article 7 of BUT Rules for Studies and Examinations.

Assesment methods and criteria linked to learning outcomes

The student must obtain at least 50 points out of 100 in the sub-activities:
1. laboratory tasks (min 10 points, 20 points)
2. individual projects, presentations (min 10 points, 20 points)
3. final exam (min 30 points, up 60 points).
Students are assessed in the laboratory, where they must demonstrate knowledge of laboratory methods discussed. Final test checks the students' knowledge of the methods and design of laboratory instruments.

Course curriculum

- The methods used in genomics and proteomics
- Laboratories, requirements for the working environment
- Fluorescence of biological molecules, fluorescent labels
- Fluorescent labeling of DNA and proteins
- Sequencing of DNA
- PCR, real-time PCR
- Chromatography general
- Liquid Chromatography
- Gas Chromatography
- Flow Cytometry
- Mass Spectrometry
- Absorption Spectrometry
- Fluorescence Spectrometry

Work placements

Not applicable.

Aims

Knowledge of information structure in biophysical methods for biological data analysis. Understanding to used methods and construction of laboratory devices.

Specification of controlled education, way of implementation and compensation for absences

Laboratory exercises are mandatory, properly excused missed lectures can be compensated individually after discussion with teacher.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Skoog,D.A.-Holler,F.J.-Nieman,T.A.: Principles of Instrumental Analysis, Saunders College Publishing, 1998, 849 s.
Skoog, D.A.-West, D.M.-Holler,F.J.: Fundamentals of Analytical chemistry, 7th edition, Saunders College Publishing, 1996, 659 s. Skoog, D.A.-West, D.M.-Holler,F.J.: Fundamentals of Analytical chemistry, 7th edition, Saunders College Publishing, 1996, 659 s.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme BTBIO-F Master's

    branch F-BTB , 2. year of study, winter semester, compulsory

  • Programme EEKR-CZV lifelong learning

    branch ET-CZV , 1. year of study, winter semester, compulsory

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer

doc. Ing. Jana Kolářová, Ph.D.

Syllabus

1. Overview of methods used in genomics and proteomics. Basic term definition.
2. Small laboratory devices.
3. DNA sequencing - sample preparation, sample separation.
4. DNA sequencing - sample preparation, PCR.
5. DNA sequencing.
6. Protein analysis - sample preparation, HPLC.
7. Flow cytometry.
8. Chromatography.
9. Spectrometry - overview of spectrometry methods.
10. Mass spectrometry, absorption spectrometry, fluorescence spectrometry.
11. Natural and non-natural fluorescence biological molecules, fluorescence marks.
12. Fluorescence marks of DNA and proteins.
13. Colorimetry.

Laboratory exercise

26 hours, compulsory

Teacher / Lecturer

Ing. Vojtěch Bartoň
doc. Ing. Jana Kolářová, Ph.D.

Syllabus

1. Laboratory introduction and organization of laboratory works.
2. Sample preparation.
3. DNA separation by gel electrophoresis.
4. Quality control of sample separation.
5. Amplification of DNA by PCR.
6. DNA sequencing of plant samples.
7. Sequencing evaluation, sample identification.
8. DNA analysis by spectrophotometer CE2502.
9. DNA analysis by spectrophotometer CE3041.
10. Protein molecules preparing.
11. Protein analysis by spectrophotometer CE2502.
12. Protein analysis by spectrophotometer CE3041.
13. Practical test.