Laboratorry Classes in Microbiology
FCH-BCO_MIB_PAcad. year: 2018/2019
The basic rules of safety work in microbiological laboratory. Preparation of culture media, various sterilization methods. Inoculation, incubation, isolation of microorganisms. Preparation of smears for staining, microscopic observation. The Gram´s staining method, several other special staining techniques. Quantification techniques - direct and indirect method. Yeasts. Fungi. The microbiological analysis of water and soil. The microbiological control of foods.
Learning outcomes of the course unit
Practical knowledge and skills of basic microbiological laboratory methods: preparation of culture medium, inoculation, incubation and isolation of microorganisms, preparation of smears for staining, various staining methods, microscopic observation of microorganisms.
The ability to apply these skills for the microbiological examination of foods, water and other materials.
Successful completion of practical classes is conditional on the basic theoretic knowledge of general microbiology.
Recommended optional programme components
Recommended or required reading
Drdák M., Veselá M.: Praktikum z obecné mikrobiologie. FCH VUT v Brně, Brno 1998. (CS)
Němec, M., Horáková, D.: Základy mikrobiologie. Vydavatelství MU, Brno 1999. (CS)
Vytřasová, J., Bílková, Z.: Laboratorní cvičení z obecné mikrobiologie. Univerzita Pardubice, Pardubice 1998. (CS)
Horáková, K., Baráthová, H., Vollek, V.: Mikrobiológia - návody na cvičenie. Slovenská vysoká škola technická v Bratislave, Bratislava 1986. (CS)
Planned learning activities and teaching methods
The course uses teaching methods in form of Laboratory exercise - 3 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.
Assesment methods and criteria linked to learning outcomes
Conditions for awarding the course unit credit: 100 % participation in laboratory classes, elaboration and submitting of reports in written form and credit test (at minimum 70 %).
Language of instruction
The basic rules of safety work in microbiological laboratory.
1. Preparation of sterile glassware and other tools
2. Preparation of culture media and its sterilization
3. Isolation, inoculation and storage of microorganisms. Cultivation conditions
4. Microscopic observation of microorganisms.
5. The negative staining, staining of bacterial capsules and spores
6. Quantification of microorganisms by direct microscopic counts. Vital staining.
7. Quantification of microorganisms by colony count method.
8. Morphology of yeasts
9. The microbiological analysis of water
10. Morphology of fungi
11. The microbiological analysis of soil
12. The microbiological control of foods
The aim of these practical classes is to make students familiar with basic procedures, methods and techniques used in microbiological laboratory.
Specification of controlled education, way of implementation and compensation for absences
Attendance is required and checked by the teacher.
Classification of course in study plans
- Programme BPCP_CHCHT Bachelor's
branch BPCO_CHMN , 3. year of study, winter semester, 2 credits, compulsory-optional
- Programme BPCP_CHTP Bachelor's
branch BPCO_BT , 3. year of study, winter semester, 2 credits, compulsory
- Programme BKCP_CHTP Bachelor's
- Programme BPCP_CHTP Bachelor's
branch BPCO_CHP , 3. year of study, winter semester, 2 credits, compulsory
- Programme CKCP_CZV lifelong learning
branch CKCO_CZV , 1. year of study, winter semester, 2 credits, compulsory