Course detail

Molecular Biotechnology

FCH-MC_MOBAcad. year: 2017/2018

The course is focused on the explanation of procedures used in molecular biotechnology for construction of genetically modified organisms with required characteristics. It explains the procedures used for gene transfer from one organism to the other with the aim to obtain an useful product including procedures used for optimalisation of gene expression and stability of recombinant proteins in procaryotic and eucaryotic cells. The impact of transgenic microorganisms, plants and animals in our life and their practical use are explained in addition to their construction.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Learning outcomes of the course unit

Theoretical aspects of various experimental techniques used in molecular biotechnology research in GMO construction and their practical use.

Prerequisites

Pass out the lectures of Molecular genetics I and II is recomended

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Assesment methods and criteria linked to learning outcomes

Examination is written (test) and oral. The student had to proof the understanding of the subject.

Course curriculum

1. Relationship between chemical production and biotechnologies.
2. Transfer of genes using recombinant DNA technology.
3. Cloning vectors and their use.
4. Transformation of cells by foreign DNA. Selection of cells with cloned gene.
5. Gene libraries. Cloning of sequences coding eucaryotic proteins.
6. Manipulation of gene expression in prokaryotes.
7. Gene expression from strong and inducible promotors.
8. Fusion proteins. Translation expression vectors. Increasing of protein stability. Integration and shuttle vectors.
9. Production of heterologous proteins in eucaryotic cells.
10.Yeast expression systems.
11. Insect and mammal expression systems.
12. Transgenic plants, their construction and use.
13. Transgenic animals, thein construction and use.

Work placements

Not applicable.

Aims

Lectures cover methodologies commonly used in molecular biotechnology in construction of transgenic organims and with the use of these organisms.

Specification of controlled education, way of implementation and compensation for absences

Attendance at lectures is not obligatory but it is recommended

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Not applicable.

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NKCP_CHTP Master's

    branch NKCO_CHTP , 1. year of study, winter semester, compulsory

  • Programme NPCP_CHTP Master's

    branch NPCO_CHTP , 1. year of study, winter semester, compulsory

  • Programme NPCP_MA Master's

    branch NPCO_MA , 1. year of study, winter semester, compulsory-optional

  • Programme CKCP_CZV lifelong learning

    branch CKCO_CZV , 1. year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hours, optionally

Teacher / Lecturer