Czech

4

Not applicable.

Theoretical aspects of various isolation and separation techniques focused on microorganism cells

Without restriction

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 1 teaching hour per week. The e-learning system (LMS Moodle) is available to teachers and students.

Oral examination

1. Stechiometry of microbial growth and material balances.
2. Properties of fluids.
3. Mechanical, chemical and enzymatic cell disruption.
4. Precipitation and salting-out odf proteins.
5.Synthesis of recombinant proteins in GMO.
6. Impovement of recombinant proteins stability synthetised in vivo.
7. Protein engineering and  site directed mutagenesis.
8. Extraction.
9. Extraction using aqueous two-phase systems.
10. Types of extractors and their design.
11. Large scale chromatographic techniques.
12. Enzymatic catalysis in anhydrous and quasi anhydrous solvents.
13. Validation of production quality.

Not applicable.

Lectures cover methods for biotechnoly product processing

Attendance at lectures is not obligatory but it is recommended. At seminars the calculations of model examples are given - attendance is obligatory.

Not applicable.

Not applicable.

Not applicable.

Not applicable.

13 hours, compulsory