Publication detail

Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma

KREMPLOVÁ, M. KREJČOVÁ, L. HYNEK, D. BARATH, P. MAJZLÍK, P. HORÁK, V. ADAM, V. SOCHOR, J. CERNEI, N. HUBÁLEK, J. VRBA, R. KIZEK, R.

Original Title

Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma

English Title

Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma

Type

journal article in Web of Science

Language

en

Original Abstract

Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of l of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined.

English abstract

Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of l of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined.

Keywords

electrochemical detection; automation; voltammetry; iron; biological sample; metallomics

RIV year

2012

Released

01.07.2012

Pages from

5893

Pages to

5909

Pages count

17

BibTex


@article{BUT92845,
  author="Monika {Kremplová} and Ludmila {Krejčová} and David {Hynek} and Peter {Barath} and Petr {Majzlík} and Vratislav {Horák} and Vojtěch {Adam} and Jiří {Sochor} and Natalia Vladimirovna {Cernei} and Jaromír {Hubálek} and Radimír {Vrba} and René {Kizek}",
  title="Automated Electrochemical Detection of Iron Ions in Erythrocytes from MeLiM Minipigs Suffering from Melanoma",
  annote="Modern analytical methods allow the detection of compounds of interest in biological samples in very low concentrations. However, some of these compounds are electro-active or provide electro-active complexes after derivatization. In this case, electrochemical detection is very useful. Stationary electrochemical analysers have a plenty of advantages, such as low costs, high sensitivity and repeatability. On the other hand, non-automated injection of samples belongs to the main disadvantages of electrochemical techniques. Due to this fact, we focused on testing of new electrochemical system enabling us to inject units of l of a sample and cooled sample-rack area. The analyser can be used for detection and quantification of a wide range of compounds including metals, peptides, proteins, nucleic acids and drugs. To test the possibilities of suggested system, concentration of iron in blood samples of MeLiM minipigs was measured. Primarily, we optimized the following experimental conditions as supporting electrolyte 0.1 M KBrO3 + 0.3 M NaOH + 0.01 M TEA of pH 11, and deposition potential of 0 V. Moreover, preparation of erythrocytes was optimized. Changes in iron content of control group (10 ug Fe ions/ml) and group of animals suffering from melanoma progression (3.5 ug Fe ions/ml) were determined.",
  chapter="92845",
  number="7",
  volume="7",
  year="2012",
  month="july",
  pages="5893--5909",
  type="journal article in Web of Science"
}