Publication detail

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

VACULOVIČOVÁ, M. HYNEK, D. ŠKUTKOVÁ, H. ADAM, V. PROVAZNÍK, I. KIZEK, R.

Original Title

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

English Title

Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction

Type

journal article in Web of Science

Language

en

Original Abstract

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

English abstract

Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.

Keywords

Brdicka reaction; Cancer; CE; Denaturation; Electrochemistry; Isoforms; Metallothionein

RIV year

2012

Released

01.01.2012

Pages from

270

Pages to

279

Pages count

10

BibTex


@article{BUT90347,
  author="Markéta {Vaculovičová} and David {Hynek} and Helena {Škutková} and Vojtěch {Adam} and Ivo {Provazník} and René {Kizek}",
  title="Structural changes in metallothionein isoforms revealed by capillary electrophoresis and Brdicka reaction",
  annote="Metallothionein (MT) as a potential cancer marker is at the center of interest and its properties, function and behaviour under various condititons is intensively studied. In the present study, two major mammalian MT isoforms (MT-1 and MT-2) were separated using capillary electrophoresis (CE) coupled with UV detector in order to describe their basic behavior. The results were confirmed by manual spectrometric measurements and by different pulse voltammetry Brdicka reaction. Based on these results, a model of MT behaviour under the conditions studied was suggested.",
  chapter="90347",
  doi="10.1002/elps.201100312",
  number="2",
  volume="33",
  year="2012",
  month="january",
  pages="270--279",
  type="journal article in Web of Science"
}