Publication detail

Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.

PROSECKÁ, E. RAMPICHOVÁ, M. VOJTOVÁ, L. TVRDÍK, D. MELČÁKOVÁ, Š. JUHASOVÁ, J. PLCNER, M. JAKUBOVÁ, R. JANČÁŘ, J. NEČAS, A. KOCHOVÁ, P. KLEPÁČEK, J. TONAR, Z. AMLER, E.

Original Title

Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.

English Title

Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.

Type

journal article - other

Language

en

Original Abstract

Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution.

English abstract

Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution.

Keywords

collagen/HA scaffold;MSCs;osteoblasts;pore size;mechanical testing

RIV year

2011

Released

19.08.2011

Publisher

WILEY PERIODICALS

Location

USA

ISBN

1549-3296

Periodical

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A

Year of study

99A

Number

2

State

US

Pages from

307

Pages to

315

Pages count

9

Documents

BibTex


@article{BUT89272,
  author="Lucy {Vojtová} and Josef {Jančář} and Alois {Nečas}",
  title="Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix.",
  annote="Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution.",
  address="WILEY PERIODICALS",
  chapter="89272",
  institution="WILEY PERIODICALS",
  number="2",
  volume="99A",
  year="2011",
  month="august",
  pages="307--315",
  publisher="WILEY PERIODICALS",
  type="journal article - other"
}